Homer1 (VesL-1) in the rat esophagus: focus on myenteric plexus and neuromuscular junction
| Autor: | J. Zimmermann, M. Raab, W. L. Neuhuber |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Histology Guinea Pigs Neuromuscular Junction Myenteric Plexus Biology Neuromuscular junction 03 medical and health sciences 0302 clinical medicine Motor Endplate Esophagus Homer Scaffolding Proteins Neuropil medicine Animals Rats Wistar Molecular Biology Myenteric plexus Nucleus ambiguus Microscopy Confocal Cell Biology Anatomy Immunohistochemistry Cell biology Rats Medical Laboratory Technology 030104 developmental biology medicine.anatomical_structure nervous system Metabotropic glutamate receptor Cholinergic Rabbits Postsynaptic density 030217 neurology & neurosurgery |
| Zdroj: | Histochemistry and cell biology. 148(2) |
| ISSN: | 1432-119X |
| Popis: | Homer1, a scaffolding protein of the postsynaptic density (PSD), enriched at excitatory synapses is known to anchor and modulate group I metabotropic glutamate receptors (mGluRs) and different channel- and receptor-proteins. Homer proteins are expressed in neurons of different brain regions, but also in non-neuronal tissues like skeletal muscle. Occurrence and location of Homer1 and mGluR5 in myenteric plexus and neuromuscular junctions (NMJ) of rat esophagus have yet not been characterized. We located Homer1 and mGluR5 immunoreactivity (-iry) in rat esophagus and focused on myenteric neurons, intraganglionic laminar endings (IGLEs) and NMJs, using double- and triple-label immunohistochemistry and confocal laser scanning microscopy. Homer1-iry was found in a subpopulation of vesicular glutamate transporter 2 (VGLUT2) positive IGLEs and cholinergic varicosities within myenteric ganglia, but neither in nitrergic nor cholinergic myenteric neuronal cell bodies. Homer1-iry was detected in 63% of esophageal and, for comparison, in 35% of sternomastoid NMJs. Besides the location in the PSD, Homer1-iry colocalized with cholinergic markers, indicating a presynaptic location in coarse VAChT/CGRP/NF200- immunoreactive (-ir) terminals of nucleus ambiguus neurons supplying striated esophageal muscle. mGluR5-iry was found in subpopulations of myenteric neuronal cell bodies, VGLUT2-ir IGLEs and cholinergic varicosities within the myenteric neuropil and NMJs of esophagus and sternomastoid muscles. Thus, Homer1 may anchor mGluR5 at presynaptic sites of cholinergic boutons at esophageal motor endplates, in a small subpopulation of VGLUT2-ir IGLEs and cholinergic varicosities within myenteric ganglia possibly modulating Ca2+-currents and neurotransmitter release. |
| Databáze: | OpenAIRE |
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