Lipidomic Analysis of Clostridium cadaveris and Clostridium fallax
| Autor: | Ziqiang Guan, Teresa A. Garrett, Howard Goldfine |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Plasmalogen Clostridium cadaveris Biochemistry Article Mass Spectrometry Clostridia 03 medical and health sciences chemistry.chemical_compound Cardiolipin Clostridium fallax Phosphatidylglycerol Phosphatidylethanolamine Clostridium 030109 nutrition & dietetics biology Molecular Structure Organic Chemistry Cell Biology biology.organism_classification Lipids Monoacylglycerol lipase 030104 developmental biology chemistry Lipidomics lipids (amino acids peptides and proteins) Chromatography Liquid |
| Popis: | The lipidomes of Clostridium fallax and Clostridium cadaveris were studied using thin-layer chromatography (TLC) and normal phase liquid chromatography/mass spectrometry (NPLC/MS). Both species contain diradylglycerol (DRG), monohexosyldiradylglycerol (MHDRG), monohexosyl monoacylglycerol (MHMAG), phosphatidylglycerol (PtdGro), and phosphatidylethanolamine (PtdEtn). DRG, MHDRG, PtdEtn, and PtdGro are present in both diacyl and alk-1-enyl acyl (plasmalogen) forms. Both species contain cardiolipin (Ptd(2)Gro), which is present in tetraacyl, monoalkenyl-triacyl, and dialkenyl-diacyl forms. Both species contain small amounts of phosphatidylcholine (PtdCho). The presence of octadecadienoic (18:2) acyl chains in some PtdCho species indicates that they arise from the medium because no 18:2 is seen in the other lipids and clostridia generally lack the capacity to synthesize polyunsaturated fatty acids. The major lipidomic differences between these two species are that C. fallax contains a glycerolacetal of plasmenylethanolamine while C. cadaveris contains an ethanolamine-phosphate-modified diacylglycerol. The significance of these lipid compositions is discussed. |
| Databáze: | OpenAIRE |
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