Characterization of the denitrifying bacterial community in a full-scale rockwool biofilter for compost waste-gas treatment
| Autor: | Yasuyuki Fukumoto, Kazuyoshi Suzuki, Tomoko Yasuda, Miyoko Waki, Kazutaka Kuroda, Dai Hanajima |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Livestock Nitrite Reductases Denitrification Microbial Consortia 030106 microbiology Nitrous Oxide Electrons engineering.material Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Denitrifying bacteria RNA Ribosomal 16S Ammonium Compounds Animals Hydrogen Sulfide Soil Microbiology Oligotropha carboxidovorans Bacteria Sewage biology Denaturing Gradient Gel Electrophoresis Compost Composting General Medicine biology.organism_classification Manure 030104 developmental biology Activated sludge Genes Bacterial Environmental chemistry Biofilter engineering Sewage treatment Oxidoreductases Filtration Biotechnology |
| Zdroj: | SC30201804040055 NARO成果DBa 著者版依頼中／Applying for the author's permission. |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-017-8398-y |
| Popis: | The potential denitrification activity and the composition of the denitrifying bacterial community in a full-scale rockwool biofilter used for treating livestock manure composting emissions were analyzed. Packing material sampled from the rockwool biofilter was anoxically batch-incubated with 15N-labeled nitrate in the presence of different electron donors (compost extract, ammonium, hydrogen sulfide, propionate, and acetate), and responses were compared with those of activated sludge from a livestock wastewater treatment facility. Overnight batch-incubation showed that potential denitrification activity for the rockwool samples was higher with added compost extract than with other potential electron donors. The number of 16S rRNA and nosZ genes in the rockwool samples were in the range of 1.64-3.27 × 109 and 0.28-2.27 × 108 copies/g dry, respectively. Denaturing gradient gel electrophoresis analysis targeting nirK, nirS, and nosZ genes indicated that the distribution of nir genes was spread in a vertical direction and the distribution of nosZ genes was spread horizontally within the biofilter. The corresponding denitrifying enzymes were mainly related to those from Phyllobacteriaceae, Bradyrhizobiaceae, and Alcaligenaceae bacteria and to environmental clones retrieved from agricultural soil, activated sludge, freshwater environments, and guts of earthworms or other invertebrates. A nosZ gene fragment having 99% nucleotide sequence identity with that of Oligotropha carboxidovorans was also detected. Some nirK fragments were related to NirK from micro-aerobic environments. Thus, denitrification in this full-scale rockwool biofilter might be achieved by a consortium of denitrifying bacteria adapted to the intensely aerated ecosystem and utilizing mainly organic matter supplied by the livestock manure composting waste-gas stream. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|