MASS-SPECTROMETRY IN MICROBIOLOGICAL PRACTICE OF SCIENTIFIC CENTRE OF OBSTETRICS, GYNECOLOGY AND PERINATOLOGY
| Autor: | L A Lyubasovskaya, Elena N. Ilina, Gennady T. Sukhikh, Vera Muravieva, T. V. Priputnevich, Melkumyan Ar |
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| Předmět: |
lactobacilli
0301 basic medicine medicine.medical_specialty Microorganism species identification 030106 microbiology maldi-tof-ms Medicine (miscellaneous) Microbiology Mycological Typing Techniques Comparative evaluation yeast fungi 03 medical and health sciences enterococci vitek2 compact30 parasitic diseases Yeast fungi Medicine Species identification staphylococci Gynecology nonfermenting gram-negative bacteria biology business.industry Obstetrics anaerobes General Medicine mass-spectrometry Ribosomal RNA biology.organism_classification QR1-502 neisseria streptococci Neisseria business enterobacteria Bacteria |
| Zdroj: | Scopus-Elsevier Журнал микробиологии, эпидемиологии и иммунобиологии, Vol 0, Iss 1, Pp 52-58 (2016) ResearcherID |
| Popis: | Aim. Comparative evaluation of species identification of microorganisms by MALDI-TOF mass-spectrometry and automatic biochemical analyzer VITEK2 Compact30. Materials and methods. Species identification of18 400 isolates of microorganisms (staphylococci, streptococci, enterococci, enterobacteria, nonfermenting gram-negative bacteria, lactobacilli, anaerobes, yeast fungi, neisseriae), isolated from vagina of pregnant and non-pregnant women and from newborns, was carried out. Identification of the isolated microorganisms was carried out by automatic bac-teriologic analyzer VITEK2 Compact30 (BioMerieux, France) and MALDI-TOF-MS analysis method on AutoflexIII (Bruker Daltonics, Germany) mass-spectrometer. Results. Comparative identification of 2005 isolates of microorganisms was carried out. Sequencing of ribosomal RNA was used as a reference method. Authenticity of species identification my MALDI-TOF-MS analysis method was: for staphylococci (95.8%), enterococci (97.5%), enterobacteria (98.4%), nonfermenting gram-negative bacteria (93.6%), P-hemolytic staphylococci (93.8%), lactobacilli (92.8%), yeast fungi (99.9%). Conclusion. Introduction of MALDI-TOF-MS analysis technology into practical work of microbiological laboratories exceeds previously used methods of microbiological testing in terms of speed, cost and authenticity of identification of a wide spectrum of microorganisms. |
| Databáze: | OpenAIRE |
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