Production of active human carbonic anhydrase II in E. coli

Autor:	Cecilia Forsman, Gity Behravan, Annika Osterman, Bengt-Harald Jonsson, Curt R. Enzell, Jan-Eric Berg, Mihály Bartók, István Pelczer, György Dombi
Rok vydání:	1988
Předmět:	General Chemical Engineering Carbonic anhydrase II Genetic Vectors Molecular Sequence Data medicine.disease_cause Plasmid Carbonic anhydrase Complementary DNA Gene expression medicine Escherichia coli Humans Amino Acid Sequence Cloning Molecular Carbonic Anhydrases chemistry.chemical_classification biology Base Sequence Chemistry Nucleic acid sequence DNA DNA Restriction Enzymes Molecular biology Recombinant Proteins Enzyme Biochemistry biology.protein
Zdroj:	Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry. 42(5)
ISSN:	0302-4369
Popis:	cDNA encoding human carbonic anhydrase II has been isolated and its nucleotide sequence determined. Expression of the isolated carbonic anhydrase gene in Escherichia coli from a plasmid containing the tac promoter yielded an active enzyme at a level of about 1% of total protein.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d9a218c001c381b0a6f3d1904327f59 https://pubmed.ncbi.nlm.nih.gov/2850697 Zobrazit plný text záznamu