Identification and characterization of ovary development-related protein EJO1 (Eri s 2) from the ovary ofEriocheir sinensisas a new food allergen
| Autor: | Tiantian She, Shaoshen Li, Yingying Zhang, Juanjuan Yan, Zixian Xu, Zhu Li Na, Huiqiang Li |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine Allergy animal structures Adolescent Brachyura medicine.medical_treatment Blotting Western Immunoblotting Dot blot Enzyme-Linked Immunosorbent Assay Shellfish Hypersensitivity medicine.disease_cause law.invention Microbiology 03 medical and health sciences Vitellogenin Allergen law medicine Animals Humans Child Aged biology Ovary Infant Hemocyanin Allergens Middle Aged biology.organism_classification medicine.disease Basophils Basophil activation Eriocheir 030104 developmental biology Child Preschool Immunology Recombinant DNA biology.protein Electrophoresis Polyacrylamide Gel Female Food Science Biotechnology |
| Zdroj: | Molecular Nutrition & Food Research. 60:2275-2287 |
| ISSN: | 1613-4125 |
| DOI: | 10.1002/mnfr.201600144 |
| Popis: | cope Crab is a major source of shellfish allergens. Most studies have focused on allergens in crab muscle (CM) rather than on allergens in crab ovary (CO). This study aimed to identify potential allergens in CO from Eriocheir sinensis. Methods and results Dot blot and immunoblotting results revealed the differential reactivity of CM and CO extracts to positive sera from patients allergic to crabs. Four CO proteins showed good specific IgE-binding activities in 2-DE/immunoblotting analysis; mass spectrometry identified the proteins as hemocyanin, vitellogenin, ovary development-related protein EJO1and EJO2. The recently identified allergen EJO1 is named ‘Eri s 2’ in the World Health Organization and International Union of Immunological Societies (WHO/IUIS) allergen nomenclature database. Recombinant Eri s 2 exhibited good reactivity to positive sera, and pre-incubation with recombinant Eri s 2 abrogated the reactivity of positive sera from two patients to CO in a dose-dependent manner. Moreover, co-incubation of recombinant Eri s 2 with patient basophils dose-dependently promoted basophil activation, confirming the biological activity of Eri s 2. Conclusion CO tissue is an important allergen source, and Eri s 2 is a new crab allergen. This study provides insights that will be useful for component-resolved diagnostics for crab allergy. |
| Databáze: | OpenAIRE |
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