| Autor: |
Camille Soun, Stephanie Thiebes, Julia K. Volke, Julian Uszkoreit, Daniel R. Engel, Franziska Hoffmann, Nirojah Vijitha, Thilo Bracht, Ferdinand von Eggeling, Barbara Sitek, Martin Eisenacher, Akanksha Dixit, Marius Horstmann, Annett Urbanek, Jenny Bottek, Anna-Lena Beerlage |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
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| DOI: |
10.1101/649137 |
| Popis: |
SUMMARYMacrophages perform essential functions during bacterial infections, such as phagocytosis of pathogens and elimination of neutrophils to reduce spreading of infection, inflammation and tissue damage. The spatial distribution of macrophages is critical to respond to tissue specific adaptations upon infections. Using a novel algorithm for correlative mass spectrometry imaging and state-of-the-art multiplex microscopy, we report here that macrophages within the urinary bladder are positioned in the connective tissue underneath the urothelium. Invading uropathogenicE.coliinduced an IL-6–dependent CX3CL1 expression by urothelial cells, facilitating relocation of macrophages from the connective tissue into the urothelium. These cells phagocytosed UPECs and eliminated neutrophils to maintain barrier function of the urothelium, preventing persistent and recurrent urinary tract infection.GRAPHICAL ABSTRACT |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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