Analysis Of Roasted And Ground Grains On The Seoul (Korea) Market For Their Contaminants Of Aflatoxins, Ochratoxin A And Fusarium Toxins By Lc-Ms/Ms

Autor: So-young Jung, Bu-chuhl Choe, Gi-young Shin, Jung-hun Kim, Young-zoo Chae
Jazyk: angličtina
Rok vydání: 2012
Předmět:
ISSN: 1278-1282
DOI: 10.5281/zenodo.1076747
Popis: A sensitive and specific method for quantitative determination of aflatoxins(B1, B2, G1,G2), deoxynivalenol, fumonisin(B1,B2), ochratoxin A, zearalenone, T-2 and HT-2 in roasted and ground grains using liquid chromatography combined with tandem mass spectrometry. A double extraction using a phosphate buffer solution followed by methanol was applied to achieve effective co extraction of 11 mycotoxins. A multitoxin immunoaffinity column for all these mycotoxins was used to clean up the extract. The LODs of mycotoxins were 0.1~6.1 μg/kg, LOQs were 0.3~18.4 μg/kg. Forty seven samples collected from Seoul (Korea) for mycotoxin contamination monitoring. The results showed that the occurrence of zearalenone and deoxynivalenol were frequent. Zearalenone was detected in all samples and deoxynivalenol was detected in 80.9 % samples in the range 0.626 ~ 29.264 μg/kg and N.D ~ 48.332 μg/kg respectively. Fumonisins and ochratoxin A were detected in 46.8% samples and 17 % samples respectively, aflatoxins and T-2/HT-2 toxins were not detected all samples.
{"references":["WHO/FAO. Safety Evaluation of certain Mycotoxins in Food, WHO\nFood Additives Series 47/FAO Food and Nutrition Paper 74, WHO:\nGeneva, 2001;1","P.Ambrosino, F. Galvano. V., Foglino, A. Logrieco, R. Fresa and A.\nRitieni, \"Supercritical fluid extraction of Beauvericin from\nmaize\",Talanta, vol. 62, 2004, pp.523-.530","M.P.E.Gonzalez,J. Mattusch and R. Wennrich, \"Stability and\ndetermination of aflatoxins by high-performance liquid chromatography\nwith amperometric detection\" J. Chromatogr,A , vol. 828, 1998, pp.\n439-444","L. Gonzalez-Osnaya, C.Cortes, J.M.Soriano, J.C.Molto and J.Manes,\n\"Occurrence of deoxynivalenol and T-2 toxin in bread and pasta\ncommercialized in Spain.\" Food chemistry, vol.124, 2011, pp.156-161.","K.F.Nielsen, U.Thrane, \"Fast methods for screening of trichothecenes in\nfungal cultures using gas chromatography- tandem mass spectrometry\"\nJ.Chromatogr. A, vol.929, 2001, pp75-87.","F.Grippi, L.Crosta, G.Aiello, M.Tolomeo, F.Oliveri, N.Gebbia,\nA.Curione \"Determination of stilbenes in Sicilian pistachio by\nhigh-performance liquid chromatographic diode array\n(HPLC-DAD/FLD) and evaluation of eventually mycotoxin\ncontamination.\" Food chemistry, vol.107, 2008, pp.483-488.","R.Krska, R.Schuhmacher, M.Grasserbauer, P.M.Scott, J. \"Determination\nof the Fusarium mycotoxin beauvericin at ╬╝ g/kg levels in corn by\nhigh-performance liquid chromatography with diode-array detection\"\nJ.Chromatogr. A, vol. 746, 1996, pp. 233-238","Anne Molinie, Virginie Faucet, Marcel Castegnaro, Annie\nPfohl-Leszkowicz. \"Analysis of some breakfast cereals on the French\nmarket for their contents of ochratoxin A, citrinin and fumonisin B1:\ndevelopment of a method for simultaneous extraction of ochratoxin A and\ncitrinin.\" Food chemistry, vol. 92, 2005, pp. 391-400","B.Giancaklo, B.Elisabetta, C.Edmondo, C.Valeriana, T.Giuseppina,\n\"Determination of ochratoxin A in eggs and target tissues of\nexperimentally drugged hens using HPLC- FLD.\" Food chemistry, vol.\n126, 2011, pp. 1278-1282\n[10] S.De Baere, J.Goossens, A.Osselaere, M.Devreese, V.Vandenbroucke,\nP.De Backer, S.Croubels. \"Quantitative determination of T-2 toxin, HT-2\ntoxin, deoxynivalenol and deepoxy-deoxynivalenol in animal body fluids\nusing LC-MS/MS detection.\"J.chromatogr B vol.879, 2001, pp.\n2403-2415.\n[11] Z.Zou, Z.He, H.Li, P.Han, J.Tang, C.Xi, Y.Li, L.Zhang, X.Li\n\" Development and application of a method for the analysis of two\ntrichothecenes:Deoxynivalenol and T-2 toxin in meat in China by\nHPLC-MS/MS\" Meat science, vol. 90, 2012, pp.613-617.\n[12] V. M. T. Lattanzio, M.Solfizzo, S.Powers, A.Visconti. \"Simultaneous\ndetermination of aflatoxins, ochratoxin A and Fusarium toxins in maize\nby liquid chromatography/tandem mass spectrometry after multitoxin\nimmunoaffinity clean up.\" Rapid commun. Mass spectrum..vol. 21. 2007.\npp.3253-3261.\n[13] International Conference on Harmonization of Technical Requirements\nfor registration of Pharmaceuticals for Human Use (ICH), Validation of\nAnalytical Procedures Methodology, Geneva, Switzerland,\nNovember .1996."]}
Databáze: OpenAIRE
Externí odkaz: