Differential contribution of individual dehydrin genes from Physcomitrella patens to salt and osmotic stress tolerance
| Autor: | Alexandra Castro, László Szabados, Marcel Bentancor, Omar Borsani, Imma Pérez Salamó, Sabina Vidal, Cecilia Ruibal, Valentina Carballo |
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| Rok vydání: | 2011 |
| Předmět: |
Osmosis
Osmotic shock Transgene Recombinant Fusion Proteins Arabidopsis Plant Science Sodium Chloride Physcomitrella patens Genes Plant Plant Roots chemistry.chemical_compound Gene Knockout Techniques Gene Expression Regulation Plant Stress Physiological Botany Genetics medicine Cell damage Abscisic acid Phylogeny Plant Proteins biology Abiotic stress Reproducibility of Results General Medicine biology.organism_classification medicine.disease Plants Genetically Modified Adaptation Physiological Bryopsida Cell biology Protein Transport chemistry Proteome Mutation Agronomy and Crop Science Subcellular Fractions |
| Zdroj: | Plant science : an international journal of experimental plant biology. 190 |
| ISSN: | 1873-2259 |
| Popis: | The moss Physcomitrella patens can withstand extreme environmental conditions including drought and salt stress. Tolerance to dehydration in mosses is thought to rely on efficient limitation of stress-induced cell damage and repair of cell injury upon stress relief. Dehydrin proteins (DHNs) are part of a conserved cell protecting mechanism in plants although their role in stress tolerance is not well understood. Four DHNs and two DHN-like proteins were identified in the predicted proteome of P. patens. Expression of PpDHNA and PpDHNB was induced by salt and osmotic stress and controlled by abscisic acid. Subcellular localization of the encoded proteins suggested that these dehydrins are localized in cytosol and accumulate near membranes during stress. Comparative analysis of dhnA and dhnB targeted knockout mutants of P. patens revealed that both genes play a role in cellular protection during salt and osmotic stress, although PpDHNA has a higher contribution to stress tolerance. Overexpression of PpDHNA and PpDHNB genes in transgenic Arabidopsis improved rosette and root growth in stress conditions, although PpDHNA was more efficient in this role. These results suggest that specific DHNs contribute considerably to the high stress tolerance of mosses and offer novel tools for genetic engineering stress tolerance of higher plants. |
| Databáze: | OpenAIRE |
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