P15INK4B gene methylation and expression in normal, myelodysplastic, and acute myelogenous leukemia cells and in the marrow cells of cured lymphoma patients
| Autor: | L. Fisher, J. Nayini, Harvey D. Preisler, Azra Raza, Biaoru Li, S. Creech, H. Chen, Parameswaran Venugopal |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Cancer Research Myeloid Lymphoma CD34 Antigens CD34 Bone Marrow Cells Cell Cycle Proteins Biology Myelogenous hemic and lymphatic diseases medicine Humans RNA Messenger Cyclin-Dependent Kinase Inhibitor p16 Aged Cyclin-Dependent Kinase Inhibitor p15 Tumor Suppressor Proteins Remission Induction Neoplasms Second Primary Hematology Methylation DNA Methylation Middle Aged medicine.disease Hematologic Diseases Lymphoproliferative Disorders Leukemia Myeloid Acute Leukemia medicine.anatomical_structure Oncology Myelodysplastic Syndromes DNA methylation Immunology Cancer research Bone marrow |
| Zdroj: | Leukemia. 15:1589-1595 |
| ISSN: | 1476-5551 0887-6924 |
| Popis: | P15INK4B methylation and expression was studied in bone marrow cells obtained from normal individuals, from patients who had been cured of lymphoma, and from patients with either MDS or AML. The level of p15 methylation was very low in normal BM cells and in CD34+ and CD34- subpopulations (0-6.5%; med, = 2.5%). P15INK4B transcripts were present in each of these cell populations. In contrast, methylation was the usual situation in MDS and AML marrows. The presence of methylation of the p15INK4B gene did not always indicate an absence of expression nor was expression always present if methylation was absent. P15INK4B methylation was studied in the marrows of nine patients (one studied twice) who had been cured of lymphoma and in whom hemopoiesis was believed to be normal. Increased methylaton was present in all 10 marrows. These data indicate that p15INK4B methylation is likely to be a very early event in the development of the secondary hematologic disorders. |
| Databáze: | OpenAIRE |
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