Optimal processing of bone marrow trephine biopsy: the Hammersmith Protocol
| Autor: | G W Stamp, Kikkeri N. Naresh, William Murray-Brown, Irvin A. Lampert, N Smith, Robert P. Hasserjian, K. Elderfield, D Lykidis, Donna Horncastle |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Pathology
medicine.medical_specialty Tissue Fixation Biopsy Haematoxylin Polymerase Chain Reaction Giemsa stain Pathology and Forensic Medicine chemistry.chemical_compound Clinical Protocols Bone Marrow Medicine Humans Fixative Bone marrow trephine Eosin medicine.diagnostic_test Staining and Labeling business.industry Bone Marrow Examination General Medicine DNA Neoplasm My Approach medicine.anatomical_structure chemistry In situ hybridisation Bone marrow business |
| Popis: | Specimens of bone marrow trephine biopsy (BMT) are transported and fixed in acetic acid-zinc-formalin fixative, decalcified in 10% formic acid-5% formaldehyde and processed with other specimens to paraffin-wax embedding. Sections, 1-microm-thick, are cut by experienced histotechnologists and used for haematoxylin and eosin, Giemsa, reticulin silver and other histological stains. Further, all immunohistochemical procedures used in the laboratory, including double immunostaining, can be used on these sections with no or minimal modifications. About 10,000 BMT specimens have been analysed using this procedure since 1997 and diseases involving the bone marrow have been classified successfully. More recently, standardised polymerase chain reaction-based analysis and mRNA in situ hybridisation studies have been conducted. Excellent morphology with good antigen, DNA and RNA preservation is offered by the Hammersmith Protocol. |
| Databáze: | OpenAIRE |
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