A strategy to sequence repetitive DNA based on partial restriction enzyme cleavage
| Autor: | Frederico G. C. Abath, Anthony A. Holder |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Genetics
Exonuclease III Base Sequence Molecular Sequence Data Bioengineering DNA DNA Restriction Enzymes General Medicine Biology Applied Microbiology and Biotechnology Biochemistry Sequencing by ligation Restriction fragment Restriction site chemistry.chemical_compound Restriction enzyme Restriction map chemistry biology.protein Restriction fragment length polymorphism Molecular Biology Repetitive Sequences Nucleic Acid Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. 53:195-197 |
| ISSN: | 1559-0291 0273-2289 |
| DOI: | 10.1007/bf02783494 |
| Popis: | The strategy to sequence repetitive DNA described in this article is based on partial restriction enzyme cleavage. It is an alternative to using nested deletion with exonuclease III or similar enzymes in which progressively more remote regions of the target DNA are brought into range for sequencing by universal primers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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