Bovine Elastin and κ-Elastin Secondary Structure Determination by Optical Spectroscopies
| Autor: | Bernard Sombret, Laurent Debelle, Pierre Legrand, Jean-Pierre Huvenne, Alain J.P. Alix, Maurice Berjot, Marie-Paule Jacob |
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| Přispěvatelé: | Laboratoire de Spectroscopies et Structures Biomoléculaires (LSSBM) (EA3305), Centre National de la Recherche Scientifique (CNRS), Laboratoire de Recherche Vasculaire Translationnelle (LVTS (UMR_S_1148 / U1148)), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 (LASIRE), Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Centrale Lille Institut (CLIL) |
| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
Circular dichroism
macromolecular substances 010402 general chemistry Spectrum Analysis Raman 01 natural sciences Biochemistry Protein Structure Secondary 03 medical and health sciences chemistry.chemical_compound Tropoelastin Spectroscopy Fourier Transform Infrared Molecule Animals Amino Acids [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Molecular Biology Protein secondary structure ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences integumentary system biology Circular Dichroism Cell Biology Elasticity 0104 chemical sciences Elastin [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biophysics Crystallography Monomer Solvation shell chemistry cardiovascular system biology.protein Cattle Macromolecule |
| Zdroj: | Journal of Biological Chemistry Journal of Biological Chemistry, 1995, 270 (44), pp.26099-26103. ⟨10.1074/jbc.270.44.26099⟩ Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 1995, 270 (44), pp.26099-26103. ⟨10.1074/jbc.270.44.26099⟩ |
| ISSN: | 0021-9258 1083-351X |
| DOI: | 10.1074/jbc.270.44.26099⟩ |
| Popis: | Elastin is the macromolecular polymer of tropoelastin molecules responsible for the elastic properties of tissues. The understanding of its specific elasticity is uncertain because its structure is still unknown. Here, we report the first experimental quantitative determination of bovine elastin secondary structures as well as those of its corresponding soluble kappa-elastin. Using circular dichroism and Fourier transform infrared and near infrared Fourier transform Raman spectroscopic data, we estimated the secondary structure contents of elastin to be approximately 10% alpha-helices, approximately 45% beta-sheets, and approximately 45% undefined conformations. These values were very close to those we had previously determined for the free monomeric tropoelastin molecule, suggesting thus that elastin would be constituted of a closely packed assembly of globular beta structural class tropoelastin molecules cross-linked to form the elastic network (liquid drop model of elastin architecture). The presence of a strong hydration shell is demonstrated for elastin, and its possible contribution to elasticity is discussed. |
| Databáze: | OpenAIRE |
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