Seroprevalence of SARS-CoV-2 Antibodies in Children and Adults in St. Louis, Missouri, USA
| Autor: | Haiyan Zhao, Ya-Nan Dai, Ann M. Gronowski, Ian B. Harvey, David Wang, Brittany K. Smith, Stephen M Roper, Jonathan E. Danis, Andrew B. Janowski, Christopher W Farnsworth, Daved H. Fremont |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Adolescent Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) 030106 microbiology serology Enzyme-Linked Immunosorbent Assay Antibodies Viral Asymptomatic Microbiology Serology Clinical Science and Epidemiology 03 medical and health sciences Young Adult 0302 clinical medicine Seroepidemiologic Studies Internal medicine medicine Seroprevalence Humans 030212 general & internal medicine Young adult Child Molecular Biology Missouri biology seroprevalence business.industry SARS-CoV-2 COVID-19 Infant St. Louis Middle Aged QR1-502 St louis Child Preschool Immunoglobulin G biology.protein Female Antibody medicine.symptom business Research Article |
| Zdroj: | mSphere, Vol 6, Iss 1 (2021) mSphere |
| ISSN: | 2379-5042 |
| DOI: | 10.1128/mSphere.01207-20 |
| Popis: | This study determined the percentages of both children and adult samples from the greater St. Louis metropolitan area who had antibodies to SARS-CoV-2 in late April to early May 2020. Reported coronavirus disease 2019 (COVID-19) case counts likely underestimate the true prevalence because mild or asymptomatic cases often go untested. Here, we use a sero-survey to estimate the seroprevalence of IgG antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the St. Louis, MO, metropolitan area in a symptom-independent manner. Five hundred three adult and 555 pediatric serum/plasma samples were collected from patients presenting to Barnes-Jewish Hospital or St. Louis Children’s Hospital between 14 April 2020 and 12 May 2020. We developed protocols for in-house enzyme-linked immunosorbent assays (ELISAs) using spike and nucleoprotein and used the assays to estimate a seroprevalence rate based on our samples. Overall IgG seropositivity was estimated to be 1.71% (95% credible interval [CI], 0.04% to 3.38%) in pediatric samples and 3.11% (95% CI, 0.92% to 5.32%) in adult samples. Seropositivity was significantly lower in children under 5 years of age than in adults, but rates between adults and children aged 5 or older were similar. Of the 176 samples tested from children under 4 years of age, none were positive. IMPORTANCE This study determined the percentages of both children and adult samples from the greater St. Louis metropolitan area who had antibodies to SARS-CoV-2 in late April to early May 2020. Approximately 1.7 to 3.1% of the tested individuals had antibodies, indicating that they had previously been infected by SARS-CoV-2. These results demonstrate that the extent of infection was about 10 times greater than the number of confirmed cases at that time. Furthermore, it demonstrated that by 5 years of age, children were infected to an extent similar to that of adults. |
| Databáze: | OpenAIRE |
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