Mifepristone alters expression of endometrial steroid receptors and their cofactors in new users of medroxyprogesterone acetate
| Autor: | Parviz Minoo, Juan C. Felix, John K. Jain, Wangrong Yang, Aimin Li |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Receptors
Steroid medicine.medical_specialty Steroid hormone receptor Medroxyprogesterone Gene Expression Estrogen receptor Medroxyprogesterone Acetate Biology Endometrium Double-Blind Method Internal medicine medicine Humans Protein Isoforms Medroxyprogesterone acetate medicine.diagnostic_test Obstetrics and Gynecology Mifepristone Placebo Effect Contraceptives Oral Synthetic Drug Combinations medicine.anatomical_structure Endocrinology Reproductive Medicine Hormone receptor Female Endometrial biopsy medicine.drug |
| Zdroj: | Fertility and Sterility. 87:8-23 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/j.fertnstert.2006.05.076 |
| Popis: | Objective To evaluate the effect of mifepristone on the expression of endometrial steroid receptors and their co-factors in depot medroxyprogesterone acetate (DMPA) users. Design A prospective, randomized, placebo-controlled trial. Setting Reproductive research center. Patient(s) Fifty healthy women with regular menstrual cycle. Intervention(s) One hundred fifty milligrams of DMPA were given every 3 months. Two pills (25 mg each) of placebo or mifepristone were administered every 14 days during the DMPA therapy. Four endometrial biopsy specimens were obtained from each patient. Main Outcome Measure(s) The expression of estrogen receptor subtypes α and β (ERα and ERβ), progesterone receptors A and B (PRAB and PRB), and androgen receptor messenger RNA and protein was detected by real-time polymerase chain reaction and immunohistochemistry, respectively. Steroid receptor coactivator 1 (SRC-1), silencing mediator for retinoid and thyroid-hormone receptors, and cell proliferation were evaluated by immunohistochemistry. Result(s) The expression of endometrial ERα, PRAB, PRB, and SRC-1 was increased significantly after 1 week of mifepristone, but the increase was no longer seen after 10 weeks. A positive correlation between endometrial ERα, PRAB, PRB, and SRC-1 production and proliferation was demonstrated. Conclusion(s) Short-term exposure of mifepristone in new starters of DMPA increases the expression of endometrial ERα, PRAB, PRB, and SRC-1 and promotes cell proliferation. Prolonged exposure to mifepristone does not alter the suppression of these receptors that are caused by DMPA and continues to result in endometrial atrophy. |
| Databáze: | OpenAIRE |
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