Fisetin effects on cell proliferation and apoptosis in glioma cells
| Autor: | Pinar Oztopcu-Vatan, Fulya Pak |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Flavonols
Apoptosis Caspase 3 Caspase 8 General Biochemistry Genetics and Molecular Biology Necrosis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor Survivin Humans Cytotoxic T cell MTT assay Cytotoxicity Cell Shape Cell Proliferation 030304 developmental biology Flavonoids 0303 health sciences Brain Neoplasms Chemistry Glioma Neoplasm Proteins Gene Expression Regulation Neoplastic 030220 oncology & carcinogenesis Cancer research Fisetin |
| Zdroj: | Zeitschrift für Naturforschung C. 74:295-302 |
| ISSN: | 1865-7125 0939-5075 |
| Popis: | This research investigated the antiproliferative effects of 1–500 μM fisetin in T98G and BEAS-2B cells by MTT assay. The IC50 of fisetin in T98G cells for 24 and 48 h were 93 and 75 μM, respectively. Apoptotic alterations of fisetin-treated T98G cells were observed by transmission electron microscopy. BEAS-2B was then used in comparison to T98G cells to determine the cytotoxic effects of fisetin. The IC50 of fisetin for 24 and 48 h were recorded as 270 and 90 μM in BEAS-2B cells, respectively. Different concentrations of fisetin were selected to determine the apoptotic and necrotic effects. Consequently, fisetin was determined to have more apoptotic effects in T98G than BEAS-2B cells, dose- and time-dependently. Moreover, fisetin was found to have cytotoxicity at lower doses in T98G cells compared to carmustine, as positive control. CASPASE 3, CASPASE 9, CASPASE 8, and BAX expressions were increased by the selected fisetin doses of 25 and 50 μM, while that of BCL-2 and survivin was reduced in T98G cells. These results will serve as an essential basis of future in vitro and in vivo studies, in the continuous search for alternative treatment agents for gliomas. |
| Databáze: | OpenAIRE |
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