A new approach to the biobleaching of flax pulp with laccase using natural mediators
| Autor: | Amanda Fillat, Teresa Vidal, J. F. Colom |
|---|---|
| Přispěvatelé: | Universitat Politècnica de Catalunya. Departament d'Enginyeria Tèxtil i Paperera, Universitat Politècnica de Catalunya. CIPAGRAF - Grup de Recerca Paperer i Gràfic |
| Rok vydání: | 2010 |
| Předmět: |
Acetosyringone
Environmental Engineering Coumaric Acids Bleach Color Bioengineering engineering.material Kappa number Syringaldehyde chemistry.chemical_compound stomatognathic system Flax Pasta de paper -- Blanqueig Enzyme Stability Food science Hydrogen peroxide Waste Management and Disposal Laccase biology Renewable Energy Sustainability and the Environment business.industry Basidiomycota Pulp (paper) Temperature Acetophenones General Medicine Pycnoporus cinnabarinus biology.organism_classification Biotechnology Enginyeria paperera::Pasta paperera::Blanqueig de pastes [Àrees temàtiques de la UPC] chemistry Benzaldehydes engineering Wood-pulp -- Bleaching Propionates business |
| Zdroj: | UPCommons. Portal del coneixement obert de la UPC Universitat Politècnica de Catalunya (UPC) Recercat. Dipósit de la Recerca de Catalunya instname |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2010.01.057 |
| Popis: | The phenols syringaldehyde (SA), acetosyringone (AS) and p-coumaric acid (PCA) were used as natural laccase mediators in combination with a laccase from Pycnoporus cinnabarinus to bleach flax fibres. Their performance was compared with 1-hydroxybenzotriazole (HBT) in terms of enzyme stability, and pulp and effluent properties. HBT and PCA were found to inactivate laccase in the absence of pulp. However, in the presence of unbleached flax pulp stability was increased; for example with PCA, laccase retained 77% of its initial activity, in contrast with complete inactivation in the absence of pulp. This suggests a protective effect of the pulp against denaturalization of the enzyme. All natural mediators resulted in a reduced kappa number after the subsequent alkaline treatment with hydrogen peroxide; the reduction being especially marked with SA (about 2 units – with respect to the control sample) and comparable to that obtained by HBT. Brightness was significantly increased by all natural mediators, but especially by AS and SA (23% with both), which performed very similarly to HBT in this respect. Natural mediators therefore might constitute an effective alternative to synthetic mediators for flax pulp biobleaching. This paper demonstrates for the first time the use of natural mediators in the laccase-assisted delignification of flax pulp and their effect on the properties of the resulting effluents. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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