Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV in southern Brazil

Autor: Renan Rangel Bonamigo, Luciane Cristina Gelatti, Ana Paula Becker, Pedro Alves d' Azevedo, Mirian Silva do Carmo, Antonio Carlos Campos Pignatari, Fernanda Matsiko Inoue, Fernanda Marques da Silva Castrucci
Rok vydání: 2013
Předmět:
Adult
DNA
Bacterial
Male
Methicillin-Resistant Staphylococcus aureus
Microbiology (medical)
Staphylococcus aureus
lcsh:Arctic medicine. Tropical medicine
Adolescent
lcsh:RC955-962
Bacterial Toxins
Leukocidin
Exotoxins
Microbial Sensitivity Tests
Skin infection
Biology
medicine.disease_cause
Microbiology
Young Adult
Leukocidins
medicine
Pulsed-field gel electrophoresis
Humans
Soft tissue infections
Child
Pathogen
SCCmec
Staphylococcal Infections
biochemical phenomena
metabolism
and nutrition
Methicillin-resistant
bacterial infections and mycoses
medicine.disease
Virology
Methicillin-resistant Staphylococcus aureus
Anti-Bacterial Agents
Bacterial Typing Techniques
Electrophoresis
Gel
Pulsed-Field
Community-Acquired Infections
Cross-Sectional Studies
Skin infections
Infectious Diseases
Multilocus sequence typing
Female
Parasitology
Brazil
Multilocus Sequence Typing
Zdroj: Revista da Sociedade Brasileira de Medicina Tropical, Volume: 46, Issue: 1, Pages: 34-38, Published: FEB 2013
Revista da Sociedade Brasileira de Medicina Tropical, Vol 46, Iss 1, Pp 34-38 (2013)
Revista da Sociedade Brasileira de Medicina Tropical v.46 n.1 2013
Revista da Sociedade Brasileira de Medicina Tropical
Sociedade Brasileira de Medicina Tropical (SBMT)
instacron:SBMT
Revista da Sociedade Brasileira de Medicina Tropical, Volume: 46, Issue: 1, Pages: 34-38, Published: JAN 2013
ISSN: 1678-9849
0037-8682
DOI: 10.1590/0037-868213022013
Popis: INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen commonly associated with nosocomial infections. However, it has also been associated with community-acquired skin and soft tissue infections (CA-MRSA). There are few data on the identification and prevalence of CA-MRSA infections in Brazil. METHODS: This is a cross-sectional study of 104 patients with community-acquired skin infections attending two health care centers in Porto Alegre, southern Brazil. MRSA isolates were characterized by molecular methods, including detection of the mecA gene by PCR, gene SCCmec typing, Panton-Valentine leukocidin (PVL) detection, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST). RESULTS: From the 104 samples, 58 Staphylococcus aureus isolates were obtained, of which five (8.6%) had a CA-MRSA-resistant profile. All five isolates had the mecA gene and amplified to SCCmec type IV. Analysis of chromosomal DNA by PFGE revealed the presence of two clusters related to international clones (OSPC and USA 300), with a Dice similarity coefficient >80%. The study was complemented by MLST, which detected three different strains: ST30, ST8, and ST45, the latter not presenting any relation with the clones compared in PFGE. CONCLUSIONS: The presence of CA-MRSA reveals an important change in the epidemiology of this pathogen and adds new elements to the knowledge of the molecular biology of infections by MRSA with SCCmec type IV in southern Brazil.
Databáze: OpenAIRE
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