NEW EXPERIMENTAL APPROACH IN BIODOSIMETRY:EX VIVOAPOPTOSIS DETECTION

Autor:	Lenka, Andrejsová, Jana, Čížková, Alžběta, Filipová, Anna, Lierová, Zuzana, Šinkorová
Rok vydání:	2022
Předmět:	Radiation Radiological and Ultrasound Technology Public Health Environmental and Occupational Health Animals Apoptosis Dose-Response Relationship Radiation Radiology Nuclear Medicine and imaging General Medicine Rats Wistar Flow Cytometry Lymphocyte Subsets Rats Retrospective Studies
Zdroj:	Radiation Protection Dosimetry. 198:590-596
ISSN:	1742-3406 0144-8420
Popis:	This study establishes a new experimental approach for retrospective biodosimetric assessment by apoptosis detection ex vivo. For this purpose, we used mononuclear blood leukocytes isolated from the peripheral blood of irradiated Wistar rats and cultured them ex vivo for posterior analysis. Using flow cytometry, we distinguished apoptotic lymphocyte subsets individual biodosimetric potential at different time periods after exposure: B-lymphocytes 6–8 h (0–7 Gy), natural killer cells 24 h (0–7 Gy) and T-lymphocytes 24 h (0–1 Gy). This novel experimental design innovates through the need of a single blood sample from irradiated individuals for a complete biodosimetric assessment.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d7a13c85da547cdc000e4623037dda0 https://doi.org/10.1093/rpd/ncac103 Zobrazit plný text záznamu