Trichloroethylene metabolism in vitro: an EPR/SPIN trapping study
| Autor: | Alasdair J. Carmichael, Linda Steel-Goodwin, Tasha L Pravecek |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
0301 basic medicine
Magnetic Resonance Spectroscopy Trichloroethylene Free Radicals Health Toxicology and Mutagenesis Radical Conjugated system Toxicology Photochemistry law.invention Nitrone Lipid peroxidation Cyclic N-Oxides 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine law Organic chemistry Animals Electron paramagnetic resonance Free Radical Formation chemistry.chemical_classification 030102 biochemistry & molecular biology Spin trapping Chemistry General Medicine Liver 030220 oncology & carcinogenesis Nitrogen Oxides Spin Labels |
| Zdroj: | Humanexperimental toxicology. 15(11) |
| ISSN: | 0960-3271 |
| Popis: | Trichloroethylene (TCE) was hypothesized to produce free radicals which could be detected using electron para magnetic resonance spectroscopy with the spin trap, PBN (α-phenyl tert-butyl nitrone). The free radicals detected following incubation of precision cut liver slices in media containing 10 mM PBN had hyperfine coupling constants aN=1.61 mT and aH=0.325 mT. There was a linear increase in free radicals detected in the bathing media when the headspace TCE concentration was increased from 2500- 10 000 p.p.m. The levels of conjugated dienes measured in the slices incubated in PBN supplemented media were less than slices exposed to TCE in incubation media without PBN. The PBN trap may act as a scavenger preventing the propagation of free radicals and inhibiting lipid peroxida tion. The experiments suggest that free radical formation by TCE leads to a concomitant increase in conjugated dienes in liver slices which may contribute to the pathological changes which occur in liver following TCE exposure. |
| Databáze: | OpenAIRE |
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