Inhibition of human MCF-7 breast cancer cells and HT-29 colon cancer cells by rice-produced recombinant human insulin-like growth binding protein-3 (rhIGFBP-3)
| Autor: | Stanley Cheung, Xiaohang Long, Lin-Lin Lan, Samuel S. M. Sun, Peter C.Y. Tong, Lizhong Liu, Qiaoquan Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Glycosylation Angiogenesis medicine.medical_treatment KDEL lcsh:Medicine Breast Neoplasms Biology Internal medicine medicine Humans Growth factor receptor inhibitor lcsh:Science Cell Proliferation Multidisciplinary Cell growth Growth factor lcsh:R Oryza Plants Genetically Modified Genetically modified rice Recombinant Proteins Insulin-Like Growth Factor Binding Protein 3 Endocrinology MCF-7 Colonic Neoplasms Cancer cell MCF-7 Cells lcsh:Q HT29 Cells Research Article |
| Zdroj: | PLoS ONE, Vol 8, Iss 10, p e77516 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Background Insulin-like growth factor binding protein-3 (IGFBP-3) is a multifunctional molecule which is closely related to cell growth, apoptosis, angiogenesis, metabolism and senescence. It combines with insulin-like growth factor-I (IGF-I) to form a complex (IGF-I/IGFBP-3) that can treat growth hormone insensitivity syndrome (GHIS) and reduce insulin requirement in patients with diabetes. IGFBP-3 alone has been shown to have anti-proliferation effect on numerous cancer cells. Methodology/Principal Findings We reported here an expression method to produce functional recombinant human IGFBP-3 (rhIGFBP-3) in transgenic rice grains. Protein sorting sequences, signal peptide and endoplasmic reticulum retention tetrapeptide (KDEL) were included in constructs for enhancing rhIGFBP-3 expression. Western blot analysis showed that only the constructs with signal peptide were successfully expressed in transgenic rice grains. Both rhIGFBP-3 proteins, with or without KDEL sorting sequence inhibited the growth of MCF-7 human breast cancer cells (65.76 ± 1.72% vs 45.00 ± 0.86%, p < 0.05; 50.84 ± 1.97% vs 45.00 ± 0.86%, p < 0.01 respectively) and HT-29 colon cancer cells (65.14 ±3.84% vs 18.01 ± 13.81%, p < 0.05 and 54.7 ± 9.44% vs 18.01 ± 13.81%, p < 0.05 respectively) when compared with wild type rice. Conclusion/Significance These findings demonstrated the feasibility of producing biological active rhIGFBP-3 in rice using a transgenic approach, which will definitely encourage more research on the therapeutic use of hIGFBP-3 in future. |
| Databáze: | OpenAIRE |
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