Serum-free Culture of Enriched Murine Haemopoietic Stem Cells II: Effects of Growth Factors and Haemin on Development
| Autor: | C M Heyworth, Cormier F, T. M. Dexter, I L Ponting |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Macrophage colony-stimulating factor
Clinical Biochemistry Biology Culture Media Serum-Free Colony-Forming Units Assay Mice Endocrinology Bone Marrow medicine Animals Progenitor cell Growth Substances Clonogenic assay Erythropoietin Cells Cultured Macrophage Colony-Stimulating Factor Granulocyte-Macrophage Colony-Stimulating Factor Cell Differentiation Cell Biology Hematopoietic Stem Cells Colony-stimulating factor Molecular biology Endothelial stem cell medicine.anatomical_structure Immunology Hemin Female Interleukin-3 Bone marrow Stem cell Cell Division Adult stem cell |
| Zdroj: | Growth Factors. 4:165-173 |
| ISSN: | 1029-2292 0897-7194 |
| DOI: | 10.3109/08977199109104812 |
| Popis: | A serum-free culture system was used to determine the effects of growth factors on the clonogenic development of a population of cells highly enriched for multipotential day 12 spleen colony forming cells (CFU-S) (FACS-BM). Under these conditions, interleukin-3 (IL-3) was found to be primarily a proliferative stimulus, the progenitor cells developing in the clonal assay systems produced colonies of morphologically undifferentiated cells for up to 20 days. No such induction of proliferation without maturation was observed with other growth factors (eg. granulocyte-macrophage colony stimulating factor (GM-CSF)). However, combinations of IL-3 plus secondary growth factors such as GM-CSF, macrophage colony stimulating factor (M-CSF), granulocyte colony-stimulating factor (G-CSF) or interleukin-1 (IL-1) led to the formation of colonies containing mature haemopoietic cells of the granulocytic, megakaryocytic or monocytic lineages. In contrast, erythroid development did not occur unless the protoporphyrin, haemin, was added to the cultures. Under these conditions mature erythroid cells were produced in cultures containing either IL-3 or GM-CSF (with or without erythropoietin (epo)). In replating experiments it was determined that the FACS-BM cells were able to generate large numbers of clonogenic cells for up to 30-40 free cultures. Such cultures, therefore, may be useful for investigating the biological and basis of the generation of clonogenic cells and of haemopoietic cell differentiation and development in response to growth factors. |
| Databáze: | OpenAIRE |
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