Salinity effect on the metabolic pathway and microbial function in phenanthrene degradation by a halophilic consortium
| Autor: | Yong Huang, Zuotao Zhang, Chongyang Wang, Hui Wang |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
lcsh:Biotechnology lcsh:QR1-502 Biophysics 010501 environmental sciences 01 natural sciences Applied Microbiology and Biotechnology lcsh:Microbiology 03 medical and health sciences chemistry.chemical_compound lcsh:TP248.13-248.65 Marinobacter Halophilic consortium Extremophile Microbial biodegradation 0105 earth and related environmental sciences Halomonas biology C23O pathway Phenanthrene biology.organism_classification Polycyclic aromatic hydrocarbons Halophile Community structure Metabolic pathway 030104 developmental biology chemistry Biochemistry Metabolic pathways Halotolerance C12O pathway Original Article |
| Zdroj: | AMB Express AMB Express, Vol 8, Iss 1, Pp 1-13 (2018) |
| ISSN: | 2191-0855 |
| DOI: | 10.1186/s13568-018-0594-3 |
| Popis: | With the close relationship between saline environments and industry, polycyclic aromatic hydrocarbons (PAHs) accumulate in saline/hypersaline environments. Therefore, PAHs degradation by halotolerant/halophilic bacteria has received increasing attention. In this study, the metabolic pathway of phenanthrene degradation by halophilic consortium CY-1 was first studied which showed a single upstream pathway initiated by dioxygenation at the C1 and C2 positions, and at several downstream pathways, including the catechol pathway, gentisic acid pathway and protocatechuic acid pathway. The effects of salinity on the community structure and expression of catabolic genes were further studied by a combination of high-throughput sequencing, catabolic gene clone library and real-time PCR. Pure cultures were also isolated from consortium CY-1 to investigate the contribution made by different microbes in the PAH-degrading process. Marinobacter is the dominant genus that contributed to the upstream degradation of phenanthrene especially in high salt content. Genus Halomonas made a great contribution in transforming intermediates in the subsequent degradation of catechol by using catechol 1,2-dioxygenase (C12O). Other microbes were predicted to be mediating bacteria that were able to utilize intermediates via different downstream pathways. Salinity was investigated to have negative effects on both microbial diversity and activity of consortium CY-1 and consortium CY-1 was found with a high degree of functional redundancy in saline environments. Electronic supplementary material The online version of this article (10.1186/s13568-018-0594-3) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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