Sequential Photodynamic Therapy with Phthalocyanine Encapsulated Chitosan-Tripolyphosphate Nanoparticles and Flucytosine Treatment against Candida tropicalis

Autor:	Kun-Hua Yu, Cheng-Ping Jheng, Cheng-I Lee, Yi-Hsuan Hsieh, Wen-Ching Chuang
Rok vydání:	2019
Předmět:	medicine.medical_treatment flucytosine Pharmaceutical Science Photodynamic therapy Drug resistance Microbiology Flucytosine Candida tropicalis Chitosan 03 medical and health sciences chemistry.chemical_compound medicine Photosensitizer Viability assay 030304 developmental biology 0303 health sciences biology 030306 microbiology Chemistry Antimicrobial biology.organism_classification phthalocianine photodynamic therapy chitosan medicine.drug
Zdroj:	Pharmaceutics Volume 11 Issue 1
ISSN:	1999-4923
DOI:	10.3390/pharmaceutics11010016
Popis:	Antibiotic resistance has become a crisis. Candida tropicalis (C. tropicalis) is one of the most highly virulent and drug-resistant pathogens. An alternative antimicrobial therapy to eradicate C. tropicalis effectively, without the risk of developing drug-resistance, is needed. Photodynamic therapy (PDT) is an alternative therapy that does not carry the risk of undesired drug resistance. To target the pathogens and to enhance the cellular penetration of the applied photosensitizer, we fabricated cationic chitosan/tripolyphosphate nanoparticles to encapsulate phthalocyanine. Our strategy promotes the uptake of phthalocyanine four-fold. This enhanced PDT can effectively inhibit planktonic C. tropicalis, such that only ~20% of C. tropicalis in the test survived but it has a limited ability to inhibit adherent C. tropicalis. Further tests with adherent C. tropicalis indicated that sequential treatment with PDT and flucytosine significantly eliminates pseudohyphae and yeast-like C. tropicalis cells. The cell viability is only ~10% after this sequential treatment. This study provides evidence of an effective therapy against drug resistant C. tropicalis, and this strategy can be potentially applied to other pathogens.
Databáze:	OpenAIRE
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