Protease-resistant PrP deposition in brain and non-central nervous system tissues of a murine model of bovine spongiform encephalopathy
| Autor: | James Hope, Robert A. Somerville, A. M. Tunstall, R. C. Moore, Christine Farquhar, J. Dornan |
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| Rok vydání: | 1996 |
| Předmět: |
PrPSc Proteins
Lymphoid Tissue animal diseases Bovine spongiform encephalopathy Immunoblotting Central nervous system Scrapie Biology Salivary Glands Incubation period Mice Protease resistant Virology Endopeptidases medicine Animals Pancreas Infectivity Brain Organ Size medicine.disease nervous system diseases Encephalopathy Bovine Spongiform Intracerebral inoculation Disease Models Animal medicine.anatomical_structure nervous system Murine model Cattle Injections Intraperitoneal |
| Zdroj: | Journal of General Virology. 77:1941-1946 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-77-8-1941 |
| Popis: | Infectivity within the central nervous system has been demonstrated by the transmission of bovine spongiform encephalopathy (BSE) from affected cattle to inbred laboratory mice. Sedimentable, protease-resistant PrP (PrPSc) has also been extracted from BSE-affected cattle brain. Both infectivity and PrPSc have been reported in the lymphoreticular tissues of sheep and mice clinically and preclinically affected with scrapie. Neither infectivity nor PrPSc has yet been detected in non-neural tissues of naturally occurring, clinical cases of BSE in cattle. We have used a murine model of BSE (301V isolate in VM/Dk mice) to investigate when and where PrPSc accumulates. PrPSc was detected both in brain and in extraneural sites prior to the onset of clinical symptoms. This murine BSE model differs, however, in four important aspects from our previously published findings for murine scrapie models: (a) PrPSc was found relatively late into the incubation period; (b) after intracerebral inoculation, PrPSc was found in brain before it was found in other tissues; (c) no PrPSc was found in most of the spleens from clinically affected animals after intracerebral inoculation; and (d) even after intra-peritoneal infection, PrPSc was detected in brain first. |
| Databáze: | OpenAIRE |
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