Local delivery of basic fibroblast growth factor (bFGF) using adsorbed silyl-heparin, benzyl-bis(dimethylsilylmethyl)oxycarbamoyl-heparin
| Autor: | Ray Tsang, Paul O. Zamora, Shigemasa Osaki, Prantika Som, Louis A. Peña |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_treatment
Basic fibroblast growth factor Biomedical Engineering Pharmaceutical Science Neovascularization Physiologic Bioengineering Nanotechnology Fibroblast growth factor Hydrophobic effect chemistry.chemical_compound Drug Delivery Systems Tissue engineering Coated Materials Biocompatible Implants Experimental medicine Animals Pharmacology Wound Healing Lactide Sutures Chemistry Heparin Growth factor Organic Chemistry Antithrombin Silanes Rats Biophysics Cattle Fibroblast Growth Factor 2 Endothelium Vascular Cell Division Biotechnology medicine.drug |
| Zdroj: | Bioconjugate chemistry. 13(5) |
| ISSN: | 1043-1802 |
| Popis: | A growth factor delivery system was developed that is based on the use of silyl-heparin, a chemically modified analogue of heparin. The silyl-heparin was adsorbed onto surfaces by hydrophobic interaction via the prosthetic unit and can then be used as a solid-phase adsorbent for bFGF. All the coating steps were performed by adsorption, a process that allowed preparation of surfaces by immersion or “dip-coating”. In this study a series of silyl-heparins were synthesized and each of the analogues found to function similar to unmodified heparin relative to their binding of antithrombin III and also the binding of bFGF. The silyl-heparins were found to be adsorbed onto a wide variety of substrates including polystyrene and lactide:glycolide copolymer. Enzyme-linked immumosorbant assay (ELISA) was used to establish that bFGF was readily bound to surface adsorbed silyl-heparin, and that the amount bound was directly related to amount offered for binding. Once adsorbed the silyl-heparin/ FGF was able to induce capillary tube formation of endothelial cells and to increase the growth of endothelial cells. When coated onto suture material and implanted in muscle, the FGF/silyl-heparin coating caused an increased density of mesenchymal cells in the area of the implant. This coating method could prove to be useful in a number of tissue engineering applications for the local delivery of FGF and other growth factors. |
| Databáze: | OpenAIRE |
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