cDNA cloning of an intracellular form of the human interleukin 1 receptor antagonist associated with epithelium
| Autor: | C F Bigler, George Martin, John S. Morris, C Hammerberg, S A Sporn, A Peace, Stephen Haskill, Glenn J. Jaffe, L. Van Le, S Fong |
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| Rok vydání: | 1991 |
| Předmět: |
Keratinocytes
Cytoplasm Sialoglycoproteins Molecular Sequence Data Gene Expression Interleukin 1 receptor type II Biology Transfection Polymerase Chain Reaction Epithelium Interleukin 20 Interleukin 26 Chlorocebus aethiops Animals Humans Amino Acid Sequence RNA Messenger Cloning Molecular RNA Processing Post-Transcriptional Receptors Immunologic Interleukin 12 receptor beta 1 subunit Multidisciplinary Base Sequence Proteins Receptors Interleukin-1 Interleukin DNA Molecular biology Interleukin 1 Receptor Antagonist Protein Interleukin 1 receptor antagonist Interleukin 1 receptor type I Intracellular Research Article Interleukin-1 |
| Zdroj: | Proceedings of the National Academy of Sciences. 88:3681-3685 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.88.9.3681 |
| Popis: | A cDNA encoding a receptor antagonist of interleukin 1 (IL-1ra), secreted from human monocytes, has recently been isolated and sequenced [Eisenberg, S. P., Evans, R. J., Arend, W. P., Verderber, E., Brewer, M. T., Hannum, C. H. & Thompson, R. C. (1990) Nature (London) 343, 341-346]. We have identified another version of this IL-1ra, which is predominantly expressed in epithelial cells. This IL-1ra lacks a leader sequence and, thus, is probably intracellular. Both proteins are derived from the same gene through use of an alternative transcriptional start site and internal splice-acceptor site. Expression of intracellular IL-1ra cDNA in COS cells demonstrated that the intracellular product specifically inhibited exogenous interleukin 1-dependent responses. Keratinocytes were shown to contain significant amounts of nonsecreted IL-1ra protein. Constitutive expression of the intracellular IL-1ra may be an intracellular defensive mechanism in exposed epithelial cells and/or may serve to regulate autocrine interleukin 1-mediated pathways of differentiation. |
| Databáze: | OpenAIRE |
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