Effect of temperature on plasmid stability and expression of cloned cellulase gene in a recombinantBacillus megaterium

Autor:	Jung Hoe Kim, Jong H. Jang, Kwang H. Son
Rok vydání:	2017
Předmět:	chemistry.chemical_classification Bacillaceae biology Bioengineering General Medicine Cellulase biology.organism_classification Applied Microbiology and Biotechnology Bacillales Molecular biology Enzyme Plasmid chemistry Gene expression biology.protein Fermentation Biotechnology Bacillus megaterium
Zdroj:	Biotechnology letters. 9(12)
ISSN:	1573-6776
Popis:	The expression of carboxymethyl cellulase gene inBacillus megaterium (pCK108) was investigated with respect to temperature in batch culture. The suboptimal temperature supporting maximal cell growth rate was determined to be 30 °C at which stability of the plasmid pCK108 could be maintained stable. However, cellular plasmid contents, production rate of cellulase of the cell, and efficiency of the gene expression increased significantly with increase of the temperature from 30 °C to 44 °C, even though the plasmid stability decreased up to 60% level at the end of the culture.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d5be50191b605d8f43dd3d3f56cc936 https://pubmed.ncbi.nlm.nih.gov/28247147 Zobrazit plný text záznamu Full text from SpringerLink