A Single Amino Acid Residue Change in the P Protein of Parainfluenza Virus 5 Elevates Viral Gene Expression▿
| Autor: | Dengyun Sun, Anthony P. Schmitt, Khalid Amine Timani, Yuan Lin, Biao He, Minghao Sun, Phuong Tieu Schmitt, Celia D. Keim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Gene Expression Regulation
Viral Viral protein Immunology Biology medicine.disease_cause Virus Replication Microbiology Respirovirus Cell Line Retinoblastoma-like protein 1 Mice Viral Proteins Virology Cricetinae Gene expression HSPA2 Protein A/G Chlorocebus aethiops medicine Animals Humans RNA Messenger Gene Vero Cells Messenger RNA Phosphoproteins Molecular biology Virus-Cell Interactions Viral replication Amino Acid Substitution Insect Science Mutation biology.protein RNA Viral HeLa Cells |
| Popis: | Parainfluenza virus 5 (PIV5) is a prototypical paramyxovirus. The V/P gene of PIV5 encodes two mRNA species through a process of pseudotemplated insertion of two G residues at a specific site during transcription, resulting in two viral proteins, V and P, whose N termini of 164 amino acid residues are identical. Previously it was reported that mutating six amino acid residues within this identical region results in a recombinant PIV5 (rPIV5-CPI−) that exhibits elevated viral protein expression and induces production of cytokines, such as beta interferon and interleukin 6. Because the six mutations correspond to the shared region of the V protein and the P protein, it is not clear whether the phenotypes associated with rPIV5-CPI− are due to mutations in the P protein and/or mutations in the V protein. To address this question, we used a minigenome system and recombinant viruses to study the effects of mutations on the functions of the P and V proteins. We found that the P protein with six amino acid residue changes (Pcpi−) was more efficient than wild-type P in facilitating replication of viral RNA, while the V protein with six amino acid residue changes (Vcpi−) still inhibits minigenome replication as does the wild-type V protein. These results indicate that elevated viral gene expression in rPIV5-CPI− virus-infected cells can be attributed to a P protein with an increased ability to facilitate viral RNA synthesis. Furthermore, we found that a single amino acid residue change at position 157 of the P protein from Ser (the residue in the wild-type P protein) to Phe (the residue in Pcpi−) is sufficient for elevated viral gene expression. Using mass spectrometry and 33 P labeling, we found that residue S157 of the P protein is phosphorylated. Based on these results, we propose that phosphorylation of the P protein at residue 157 plays an important role in regulating viral RNA replication. |
| Databáze: | OpenAIRE |
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