Mapping genetic determinants for human immunodeficiency virus type 1 resistance to soluble CD4
| Autor: | I. S. Y. Chen, E. S. Daar, D. D. Ho, William A. O'Brien |
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| Rok vydání: | 1992 |
| Předmět: |
viruses
Immunology Biology HIV Envelope Protein gp120 Microbiology Polymerase Chain Reaction Epitope Virus Neutralization law.invention Antigen-Antibody Reactions Epitopes Viral envelope law Virology Humans Cloning Molecular Tropism Infectivity Recombination Genetic Acquired Immunodeficiency Syndrome Binding Sites Strain (chemistry) virus diseases Chromosome Mapping Organ Specificity Insect Science CD4 Antigens Recombinant DNA HIV-1 Research Article |
| Zdroj: | Journal of virology. 66(5) |
| ISSN: | 0022-538X |
| Popis: | Neutralization of human immunodeficiency virus type 1 (HIV-1) infection with soluble CD4 (sCD4) can be achieved over a broad range of concentrations for different virus strains. Laboratory virus strains passaged in transformed T-cell lines are typically sensitive to sCD4 neutralization, whereas primary virus isolates require over 100-fold-higher sCD4 concentrations. Using recombinant viruses generated from a laboratory strain, HIV-1NL4-3, and a primary macrophagetropic strain, HIV-1JR-FL, we mapped a region of gp120 important for determining sensitivity to sCD4 neutralization. This same region has previously been defined as important for macrophage and transformed T-cell line tropism and includes the V3 neutralization domain but does not include regions of gp120 that have been shown to be most important for CD4 binding. |
| Databáze: | OpenAIRE |
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