Preparation of Rat Tail Tendons for Biomechanical and Mechanobiological Studies
| Autor: | Nadia Champagne, Gabriel Parent, Eve Langelier, Paule Cousineau-Pelletier, Amélie Bruneau |
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| Rok vydání: | 2010 |
| Předmět: |
Tail
General Immunology and Microbiology Computer science Biological modeling General Chemical Engineering General Neuroscience Biomechanics Bioengineering Anatomy musculoskeletal system Rat tail Area measurement General Biochemistry Genetics and Molecular Biology Biomechanical Phenomena Rats Tendons Mechanobiology Rat tail tendon Animals Biomedical engineering Tissue viability |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| Popis: | Rat tail tendons (RTTs) are a common biological model used in experimental in vitro studies in the fields of tendon physiology and tendinopathy. Working with those tissues is challenging because they are very fragile, and until now there was no rigorously detailed protocol for their isolation. Faced with these challenges, we have developed methods and instruments to facilitate manipulation of RTTs and control tissue viability, sterility and integrity. This article describes the experimental procedures used to prepare RTTs for biomechanical and mechanobiological studies. Our work is divided into four main steps: extraction, cross-sectional area measurement, rinsing and loading into the bioreactor chamber. At each step, all procedures, materials and manipulations are presented in detail so that they can be easily reproduced. Moreover, the specific instruments developed are presented: a manipulation plate used to segregate RTTs, an optic micrometer to position the tissue during the cross-sectional area measurement and an anchoring system to attach the RTTs onto a bioreactor. Finally, we describe the results obtained after multiple tests to validate our methods. The viability, sterility and integrity evaluations demonstrate that our procedures are sufficiently rigorous for manipulations of fragile tissues such as rat tail tendons. |
| Databáze: | OpenAIRE |
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