Characterization of the murine β-hexosaminidase (HEXB) gene
| Autor: | Jacquetta M. Trasler, Barbara Triggs-Raine, Timothy J. Salo, Guylaine Benoit, Roy A. Gravel |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Molecular Sequence Data Biology Homology (biology) Cell Line Mice Hexosaminidase B Animals Coding region Amino Acid Sequence RNA Messenger Molecular Biology Gene Genetics Messenger RNA Reporter gene Base Sequence Intron Sandhoff Disease Exons Haplorhini Cosmids Molecular biology Introns beta-N-Acetylhexosaminidases HEXB Cosmid Molecular Medicine Sequence Alignment |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1227:79-86 |
| ISSN: | 0925-4439 |
| DOI: | 10.1016/0925-4439(94)90110-4 |
| Popis: | The murine HEXB gene, encoding the β-subunit of the lysosomal hydrolase, β-hexosaminidase, was isolated from a mouse cosmid library as a single cosmid clone. The entire gene spans 22 kb, considerably less than the 40 kb spanned by its human counterpart. It is highly homologous to the human gene. The 14 intron-exon junctions are entirely conserved, although the intron sequences diverge rapidly. Upstream of the coding region, a 1.3 kb segment was sequenced and shown to function as a promoter when fused with a reporter gene and expressed in monkey COS-7 cells. A short sequence (100 bp), near the start of the coding region, exhibits strong homology to the human HEXB promoter. Analysis of the tissue distribution of the HEXB mRNA in 129/Sv male mice revealed up to 28-fold tissue-specific variations in transcript levels. The kidney and the epididymis had the highest mRNA levels consistent with past surveys of enzyme activity. |
| Databáze: | OpenAIRE |
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