The CXC Chemokine Receptors in Four-Eyed Sleeper (Bostrychus sinensis) and Their Involvement in Responding to Skin Injury
| Autor: | Hong Zhang, Meisheng Yi, Wenjing Li, Dong Mengdan, Chengyu Mo, Wanwan Zhang, Wei Liu, Kuntong Jia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
QH301-705.5 Biology Catalysis Article Microbiology Inorganic Chemistry Immune system Aquaculture Phylogenetics medicine Animals Humans CXC chemokine receptors Physical and Theoretical Chemistry Nuclear membrane Biology (General) Molecular Biology Gene QD1-999 Spectroscopy Receptors CXCR6 Skin Receptors CXCR business.industry four-eyed sleeper Organic Chemistry General Medicine biology.organism_classification Bostrychus CXCR6 Computer Science Applications Chemistry medicine.anatomical_structure HEK293 Cells Female skin injury Wound healing business Transcriptome |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 18 International Journal of Molecular Sciences, Vol 22, Iss 10022, p 10022 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms221810022 |
| Popis: | CXC Chemokine signaling plays an important role in wound healing. The four-eyed sleeper (Bostrychus sinensis) is a commercially important marine fish, which is prone to suffer skin ulceration at high temperature seasons, leading to mass mortality of fish in aquaculture farms. The genetic background related to skin ulceration and wound healing has remained unknown in this fish. Herein, we identified 10 differentially expressed Bostrychus sinensis CXC chemokine receptors (BsCXCRs) in skin ulcerated fish by de novo transcriptome sequencing. The transcripts of these BsCXCRs were classified in seven types, including BsCXCR1a/1b, BsCXCR2, BsCXCR3a1/3a2, BsCXCR4a/4b, and BsCXCR5-7, and BsCXCR6 was the first CXCR6 homologue experimentally identified in teleost fish. These BsCXCRs were further characterized in gene and protein structures, as well as phylogenetics, and the results revealed that BsCXCRs have expanded to divergent homologues. Our results showed that, in healthy fish, the BsCXCR transcripts was mainly distributed in the muscle and immune related organs, and that BsCXCR1a/1b proteins located in the cytomembrane, BsCXCR4a/4b/5/6 in the cytomembrane and perinuclear region, and BsCXCR3a1/3a2/7 in the cytomembrane, perinuclear region, and nuclear membrane, respectively. In skin injured fish, the transcripts of all BsCXCRs were transiently increased within one hour after injury, suggesting the involvement of BsCXCRs into the early inflammatory response to skin injury in the four-eyed sleeper. These results are valuable for understanding the evolutionary events of fish CXCR genes and provide insights into the roles of CXCR family in fish skin injury. |
| Databáze: | OpenAIRE |
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