Impairment of Polymorphonuclear Neutrophil Function in HIV-Infected Patients
| Autor: | Carole Elbim, F. Bouscarat, M. A. Gougerot-Pocidalo, S. Chollet-Martin, E. Franzini, Marie-Hélène Prevot, Jacques Hakim |
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| Rok vydání: | 1995 |
| Předmět: |
Adult
Male Neutrophils HIV Infections CD18 Biology medicine.disease_cause Neutrophil Activation In vivo medicine Humans Pharmacology Cell adhesion molecule Hydrogen Peroxide Flow Cytometry Actins CD4 Lymphocyte Count Respiratory burst N-Formylmethionine Leucyl-Phenylalanine Integrin alpha M Immunology Selectins biology.protein Female Tumor necrosis factor alpha Cardiology and Cardiovascular Medicine Cell Adhesion Molecules Ex vivo Oxidative stress |
| Zdroj: | Journal of Cardiovascular Pharmacology. 25:S66-S70 |
| ISSN: | 0160-2446 |
| DOI: | 10.1097/00005344-199500252-00014 |
| Popis: | Impaired polymorphonuclear neutrophil (PMN) function may contribute to the onset of certain bacterial and fungal infections and to tissue damage in human immunodeficiency virus (HIV)-infected patients. Published data on PMN function in HIV infection are controversial, possibly because most studies have involved PMNs isolated from the normal blood environment by various procedures that may modify PMN responses. We therefore used flow cytometry to study the expression of adhesion molecules at the PMN surface, actin polymerization, and the oxidative burst of whole-blood PMNs in 42 HIV-infected patients at different stages of the disease. These PMNs were activated in vivo, as shown by increased expression of the adhesion molecule CD11b/CD18, reduced L-selectin antigen expression, increased actin polymerization, and increased H2O2 production. The alterations were present in asymptomatic patients with CD4+ cell counts above 500/microliters and did not increase with progression of the disease. This PMN activation could contribute to the oxidative stress described in HIV infection. Stimulation by bacterial N-formyl peptides showed dysregulation of L-selectin shedding and decreased H2O2 production after cx vivo priming with tumor necrosis factor-alpha or interleukin-8. These latter impairments, which correlated with the decrease in CD4+ lymphocyte numbers, could contribute to the increased susceptibility of HIV-infected patients to bacterial infections. |
| Databáze: | OpenAIRE |
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