Comparative methylation profiles and telomerase biology of mouse multipotent adult germline stem cells and embryonic stem cells
| Autor: | Katayoon Shirneshan, Britta Kaltwasser, Ulrich Zechner, Jessica Nolte, Thomas Haaf, Marieke Wolf, Daniela Weise, Wolfgang Engel, Nady El Hajj, Athanasios Zovoilis |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Embryology Adult Germline Stem Cells Telomerase Somatic cell Biology Polymerase Chain Reaction Germline 03 medical and health sciences Mice 0302 clinical medicine Genetics Animals Molecular Biology Cells Cultured Embryonic Stem Cells 030304 developmental biology 0303 health sciences Multipotent Stem Cells Obstetrics and Gynecology Cell Differentiation Cell Biology DNA Methylation Molecular biology Embryonic stem cell 3. Good health Reproductive Medicine 030220 oncology & carcinogenesis DNA methylation Electrophoresis Polyacrylamide Gel Female Stem cell Genomic imprinting Developmental Biology |
| Zdroj: | Molecular human reproduction. 15(6) |
| ISSN: | 1460-2407 |
| Popis: | Recently, several groups described the isolation of mouse spermatogonial stem cells (SSCs) and their potential to develop to embryonic stem cell (ESC)-like cells, so-called multipotent germline stem cells (mGSCs). We were the first to derive such mGSCs from SSCs isolated from adult mouse testis and, therefore, called these mGSCs multipotent adult germline stem cells (maGSCs). Here, we compara- tively analyzed gene-specific and global DNA methylation profiles as well as the telomerase biology of several maGSC and male ESC lines. We show that undifferentiated maGSCs are very similar to undifferentiated male ESCs with regard to global DNA methylation, methylation of pluripotency marker gene loci, telomerase activity and telomere length. Imprinted gene methylation levels were generally lower in undif- ferentiated maGSCs than in undifferentiated male ESCs, but, compared with undifferentiated mGSCs derived by other groups, more similar to those of male ESCs. Differentiation of maGSCs increased the methylation of three of the four analyzed imprinted genes to almost somatic methylation patterns, but dramatically decreased global DNA methylation. Our findings further substantiate the pluripotency of maGSCs and their potential for regenerative medicine. |
| Databáze: | OpenAIRE |
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