Nanopillared Chitosan/Gelatin Films: A Biomimetic Approach for Improved Osteogenesis
Autor: | Sevde Altuntas, Thomas J. Webster, Pınar Alpaslan, Harkiranpreet Kaur Dhaliwal, Mansoor M. Amiji, Fatih Buyukserin, Nicole J. Bassous, Ahmed E. Radwan |
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Přispěvatelé: | TOBB ETU, Faculty of Engineering, Department of Biomedical Engineering, TOBB ETÜ, Mühendislik Fakültesi, Biyomedikal Mühendisliği Bölümü, Büyükserin, Fatih |
Rok vydání: | 2021 |
Předmět: |
food.ingredient
0206 medical engineering Biomedical Engineering nanopillared chitosan/gelatin film 02 engineering and technology Bone tissue Gelatin Osseointegration Biomaterials Chitosan osteogenic differentiation chemistry.chemical_compound food medicine Nanotopography Bone regeneration Nanoporous Mesenchymal stem cell technology industry and agriculture mineralization 021001 nanoscience & nanotechnology 020601 biomedical engineering medicine.anatomical_structure chemistry Chemical engineering 0210 nano-technology |
Zdroj: | ACS biomaterials scienceengineering. 5(9) |
ISSN: | 2373-9878 |
Popis: | Biomimicry strategies, inspired from natural organization of living organisms, are being widely used in the design of nanobiomaterials. Particularly, nonlithographic techniques have shown immense potential in the facile fabrication of nanostructured surfaces at large-scale production. Orthopedic biomaterials or coatings possessing extracellular matrix-like nanoscale features induce desirable interactions between the bone tissue and implant surface, also known as osseointegration. In this study, nanopillared chitosan/gelatin (C/G) films were fabricated using nanoporous anodic alumina molds, and their antibacterial properties as well as osteogenesis potential were analyzed by comparing to the flat C/G films and tissue culture polystyrene as controls. In vitro analysis of the expression of RUNX2, osteopontion, and osteocalcin genes for mesenchymal stem cells as well as osteoblast-like Saos-2 cells was found to be increased for the cells grown on nano C/G films, indicating early-stage osteogenic differentiation. Moreover, the mineralization tests (quantitative calcium analysis and alizarin red staining) showed that nanotopography significantly enhanced the mineralization capacity of both cell lines. This work may provide a new perspective of biomimetic surface topography fabrication for orthopedic implant coatings with superior osteogenic differentiation capacity and fast bone regeneration potential. Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [1059B141601323] |
Databáze: | OpenAIRE |
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