Nuclear and cytoplasmic quality of oocytes derived from serum-free culture of secondary follicles in vitro
| Autor: | Ya‐Ping Shi, Chun-Ying Song, Feng Han, Qing-Yuan Sun, Jian Li, Zheng-Hui Zhao, Feng Wang, Li-Hua Fan, Xing-Ping Guo, Wen-Long Lei, Qian Zhou, Ang Li, Huaixiu Wang, Heide Schatten |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cytoplasm Physiology Clinical Biochemistry Reproductive technology Biology Andrology 03 medical and health sciences Mice 0302 clinical medicine Meiosis Ovarian Follicle In vivo Follicular phase Animals Cell Nucleus Embryogenesis Cell Biology Antral follicle Embryonic stem cell In vitro In Vitro Oocyte Maturation Techniques 030104 developmental biology 030220 oncology & carcinogenesis Oocytes Female |
| Zdroj: | Journal of cellular physiologyREFERENCES. 236(7) |
| ISSN: | 1097-4652 |
| Popis: | In vitro culture of follicles is a promising technology to generate large quantities of mature oocytes and it could offer a novel option of assisted reproductive technologies. Here we described a 2-dimensional follicular serum-free culture system with 3-dimensional effect that can make secondary follicles develop into antral follicles (78.52%), generating developmentally mature oocytes in vitro (66.45%). The oocytes in this serum-free system completed the first meiosis; spindle assembly and chromosome congression in most oocytes matured from follicular culture were normal. However, these oocytes showed significantly lower activation and embryonic development rates, and their ability to produce Ca2+ oscillations was also lower in response to parthenogenetic activation, after which a 2-cell embryonic developmental block occurred. Oocytes matured from follicular culture displayed increased abnormal mitochondrial distribution and increased reactive oxygen species levels when compared to in vivo matured oocytes. These data are important for understanding the reasons for reduced developmental potential of oocytes matured from follicular culture, and for further improving the cultivation system. |
| Databáze: | OpenAIRE |
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