Cause or Effect? Stretching to Understand the Inflammatory Role of Elastin Fiber Breakdown in Chronic Obstructive Pulmonary Disease
| Autor: | Sara J Bonvini |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Dietary Fiber
Lipopolysaccharides Male Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Clinical Biochemistry Pulmonary disease Elastin fiber Desmosine Pulmonary Disease Chronic Obstructive Text mining Leukocytes Humans Animals Medicine Lung Molecular Biology Inflammation Mesocricetus business.industry Chemotaxis Isodesmosine Editorials Cell Biology Elastic Tissue Elastin Female Peptides business Bronchoalveolar Lavage Fluid |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology |
| ISSN: | 1535-4989 1044-1549 |
| Popis: | The mechanisms responsible for the increased loss of pulmonary function following acute lung inflammation in chronic obstructive pulmonary disease remain poorly understood. To investigate this process, our laboratory developed a hamster model that uses a single intratracheal instillation of LPS to superimpose an inflammatory response on lungs treated with intratracheal elastase 1 week earlier. Parameters measured at 2 days after LPS included total leukocyte content and percent neutrophils in BAL fluid (BALF), and BALF levels of both total and peptide-free elastin-specific crosslinks, desmosine and isodesmosine (DID). Airspace enlargement, measured by the mean linear intercept method, and relative interstitial elastic fiber surface area were determined at 1 week after LPS. Compared with animals only treated with elastase, those receiving elastase/LPS showed statistically significant increases in mean linear intercept (156.2 vs. 85.5 μm), BALF leukocytes (187 vs. 37.3 × 10 |
| Databáze: | OpenAIRE |
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