Overexpression of Stat3 increases circulating cfDNA in breast cancer
| Autor: | Yi Ding, Yi-Fei Wang, Yu Jiang, Xuejian Wang, Li-Hong Shi, Zhong Lu, Li-Hua Wang, Cong-Cong Cheng, Shu-Rong Liu, Xiao-Qing Wan |
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| Rok vydání: | 2021 |
| Předmět: |
STAT3 Transcription Factor
0301 basic medicine Cancer Research Epithelial-Mesenchymal Transition Breast Neoplasms Immunofluorescence Metastasis Mice 03 medical and health sciences 0302 clinical medicine Circulating tumor cell Breast cancer In vivo Biomarkers Tumor medicine Animals Humans Epigenetics Liquid biopsy medicine.diagnostic_test Oncogene business.industry Liquid Biopsy Neoplastic Cells Circulating medicine.disease 030104 developmental biology Oncology 030220 oncology & carcinogenesis Cancer research Female business Cell-Free Nucleic Acids |
| Zdroj: | Breast Cancer Research and Treatment. 187:69-80 |
| ISSN: | 1573-7217 0167-6806 |
| Popis: | Current studies on circulating cell-free DNA (cfDNA) have been focusing on its potential as biomarkers in liquid biopsy by detecting its content or genetic and epigenetic changes for the evaluation of tumor burden and therapeutic efficacy. However, the regulatory mechanism of cfDNA release remains unclear. Stat3 has been documented as an oncogene for the development and metastasis of breast cancer cells. In this study, we investigated whether Stat3 affects the release of cfDNA into blood and its association with the number of circulating tumor cells (CTCs). The cfDNA level in plasma of patients with breast cancer and healthy volunteers were determined by quantitative real-time PCR. Three mouse breast cancer models with different Stat3 expression were generated and used to established three breast cancer orthotopic animal models to examine the effect of Stat3 on cfDNA release in vivo. Stat3 mediated Epithelial-mesenchymal phenotype transition of CTCs was determined by immunofluorescence assay and Western blot assay. The data showed that Stat3 increased circulating cfDNA, which is correlated with the increased volume of primary tumors and number of CTCs, accompanied with the dynamic EMT changes regulated by Snail induction. Furthermore, the high level of total circulating cfDNA and Stat3-cfDNA in patients with breast cancer were detected by quantitative real-time PCR using GAPDH and Stat3 primers. Our results suggested that Stat3 increases the circulating cfDNA and CTCs in breast cancer. |
| Databáze: | OpenAIRE |
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