Early postnatal exposure of mice to side-steam tobacco smoke increases neuropeptide Y in lung
Autor: | K. B. Benders, Dawn D. Hunter, Richard D. Dey, Zhong-Xin Wu |
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Rok vydání: | 2012 |
Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Physiology Carbazoles Neuropeptide Biology Indole Alkaloids Mice chemistry.chemical_compound Nerve Fibers Airway resistance Neurotrophic factors Physiology (medical) Internal medicine Nerve Growth Factor medicine Animals Neuropeptide Y Methacholine Chloride Mice Inbred ICR Lung Airway Resistance Age Factors Muscle Smooth Articles Cell Biology Protein-Tyrosine Kinases respiratory system Neuropeptide Y receptor Asthma Trachea medicine.anatomical_structure Endocrinology Nerve growth factor Animals Newborn chemistry Tobacco Smoke Pollution K252a Tyrosine kinase |
Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 302:L152-L159 |
ISSN: | 1522-1504 1040-0605 |
Popis: | Our recent study showed that prenatal and early postnatal exposure of mice to side-steam tobacco smoke (SS), a surrogate to environmental tobacco smoke (ETS), leads to increased airway responsiveness and sensory innervation later in life. However, the underlying mechanism initiated in early life that affects airway responses later in life remains undefined. The concomitant increase in nerve growth factor (NGF) after exposures suggests that NGF may be involved the regulation of airway innervation. Since NGF regulates sympathetic nerve responses, as well as sensory nerves, we extended previous studies by examining neuropeptide Y (NPY), a neuropeptide associated with sympathetic nerves. Different age groups of mice, postnatal day (PD) 2 and PD21, were exposed to either SS or filtered air (FA) for 10 consecutive days. The level of NPY protein in lung and the density of NPY nerve fibers in tracheal smooth muscle were significantly increased in the PD2–11SS exposure group compared with PD2–11FA exposure. At the same time, the level of NGF in lung tissue was significantly elevated in the PD2–11SS exposure groups. However, neither NPY (protein or nerves) nor NGF levels were significantly altered in PD21–30SS exposure group compared with the PD21–30FA exposure group. Furthermore, pretreatment with NGF antibody or K252a, which inhibits a key enzyme (tyrosine kinase) in the transduction pathway for NGF receptor binding, significantly diminished SS-enhanced NPY tracheal smooth muscle innervation and the increase in methacholine-induced airway resistance. These findings show that SS exposure in early life increases NPY tracheal innervation and alters pulmonary function and that these changes are mediated through the NGF. |
Databáze: | OpenAIRE |
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