Prevalence of non-organ-specific autoantibodies and chronic liver disease in the general population: a nested case-control study of the Dionysos cohort
| Autor: | G. Saccoccio, Marco Lenzi, Francesco B. Bianchi, Stefano Bellentani, Fabio Cassani, Claudio Tiribelli, Paolo Muratori, F Masutti, Luigi Muratori |
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| Rok vydání: | 1999 |
| Předmět: |
Hepatitis
education.field_of_study HBsAg business.industry Hepatitis C virus Population Gastroenterology Hepatitis C Chronic liver disease medicine.disease medicine.disease_cause Article Immunology Nested case-control study medicine Humans education business Prospective cohort study Biomarkers Autoantibodies |
| Zdroj: | Gut. 45:435-441 |
| ISSN: | 1468-3288 0017-5749 |
| DOI: | 10.1136/gut.45.3.435 |
| Popis: | BACKGROUNDSeveral retrospective and prospective studies report an increased prevalence of non-organ-specific autoantibodies (NOSAs) in patients with hepatitis C virus (HCV) related chronic liver disease (CLD). Some of the data so far available are controversial and the true prevalence of NOSAs in the general population is still not known.AIMTo explore the prevalence of NOSAs, their relation to different HCV genotypes, and the presence and severity of CLD in the general population of Northern Italy.PATIENTSAll 226 anti-HCV positive and 87 hepatitis B surface antigen (HBsAg) positive patients of the Dionysos cohort study were analysed and compared with sex and age matched cases (226) negative for both anti-HCV antibody and HBsAg selected from the same cohort.METHODSSera tested for the presence of NOSAs (anti-nuclear antibody (ANA), anti-smooth muscle antibody (SMA), and anti-liver/kidney microsomes type 1 antibody (LKM1)) were screened by indirect immunofluorescence at a 1:40 serum dilution. HCV RNA and HCV genotypes were also determined by nested polymerase chain reaction (PCR) of the 5′ non-coding region and by PCR amplification of the core region with type specific primers.RESULTSThe overall prevalence of NOSA reactivity was significantly higher in anti-HCV positive subjects than in both normal and pathological controls (25%v 6% and 7% respectively, pCONCLUSIONSIn the general population the prevalence of NOSAs is higher in anti-HCV positive subjects than in normal or disease controls. Moreover NOSAs are associated with CLD and with a more active disease in terms of alanine aminotransferase activity. |
| Databáze: | OpenAIRE |
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