Novel dual-reporter transgenic rodents enable cell tracking in animal models of stem cell transplantation
| Autor: | Yasuaki Shirayoshi, Kohei Fukuoka, Kumi Morikawa, Yoshiko Suyama, Shunjiro Yagi, Kazuomi Nakamura, Kenshiro Yamamoto, Tetsuya Ohbayashi, Ichiro Hisatome |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Genetically modified mouse Luminescence MSCs mesenchymal stem cells Transgene Biophysics Stem cells Biology Biochemistry Regenerative medicine Fluorescence Green fluorescent protein EGFP enhanced green fluorescence protein lcsh:Biochemistry 03 medical and health sciences 0302 clinical medicine In vivo Tg transgenic lcsh:QD415-436 Luciferase lcsh:QH301-705.5 Reporter GFP green fluorescence protein Transplantation ADSCs adipose derived stem cells FCM flow cytometry Cell biology 030104 developmental biology lcsh:Biology (General) 030220 oncology & carcinogenesis In vivo imaging RT room temperature Stem cell Research Article |
| Zdroj: | Biochemistry and Biophysics Reports Biochemistry and Biophysics Reports, Vol 18, Iss, Pp-(2019) |
| ISSN: | 2405-5808 |
| Popis: | In the present study, we have established a novel transgenic mouse and transgenic rats with dual reporters of EGFP and ELuc. In these transgenic (Tg) rodents, both GFP fluorescent and luciferase luminescent signals were ubiquitously detected in the heart, liver, kidney and testis, while only the GFP signal was detected in the brain. This expression system is based on a P2A linked EGFP/ELuc protein allowing both signals to be generated simultaneously. Microscopy experiments, FCM, and luciferase assays showed strong expression in freshly isolated ADSCs from Tg rodents upon transplantation of Tg rat-derived ADSCs into wild-type-mice. The ELuc transgene signal was observed and traced in vivo, and EGFP positive cells could be recovered from ELuc positive tissues in engraftment sites of wild-type mice for multiple analysis. These dual reporter Tg rodents are a useful reconstituted model system of regenerative medicine and are a valuable tool to study stem cells. Highlights • Establishment of dual reporter transgenic mice and rats, which express luciferase and GFP in all organs. • Both luciferase and GFP signals were detected by in vivo imaging using their respective antibodies. • Isolated mesenchymal stem cells from transgenic rodents showed both luciferase and GFP signals. • Implantation of transgenic mesenchymal stem cells enables cell tracking in vivo. |
| Databáze: | OpenAIRE |
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