Cytotoxic T-lymphocyte responses to cytomegalovirus in normal and simian immunodeficiency virus-infected rhesus macaques
| Autor: | Amitinder Kaur, R P Johnson, D. Hempel, M S Hirsch, David Lee-Parritz, M D Daniel |
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| Rok vydání: | 1996 |
| Předmět: |
Immunology
Simian Acquired Immunodeficiency Syndrome Cytomegalovirus CD8-Positive T-Lymphocytes Biology Antibodies Viral medicine.disease_cause Microbiology Immediate early protein Cell Line Immediate-Early Proteins Immune system Antigen Virology medicine Animals Humans Cytotoxic T cell Antigens Viral Cell Line Transformed Histocompatibility Antigens Class I virus diseases Simian immunodeficiency virus Macaca mulatta CTL Insect Science Cytomegalovirus Infections Simian Immunodeficiency Virus CD8 Research Article T-Lymphocytes Cytotoxic |
| Zdroj: | Journal of Virology. 70:7725-7733 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | Disseminated cytomegalovirus (CMV) infection is a frequent occurrence in human immunodeficiency virus-infected humans and in simian immunodeficiency virus (SIV)-infected rhesus macaques. Rhesus macaques are a suitable animal model with which to study in vivo interactions between CMV and AIDS-associated retroviruses. Since cytotoxic T lymphocytes (CTL) play a major role in control of viral infections, we have characterized CMV-specific CTL responses in SIV-infected and uninfected rhesus macaques. Autologous fibroblasts infected with rhesus CMV were used to stimulate freshly isolated peripheral blood mononuclear cells from CMV-seropositive animals. Following in vitro stimulation, specific CTL activity against CMV-infected autologous fibroblasts was detected in CMV-seropositive but not in CMV-seronegative normal macaques. CMV-specific CTL activity comparable to that in normal animals was also detected in two CMV-seropositive macaques infected with a live attenuated SIV strain (SIVdelta3) and in two of three macaques infected with pathogenic SIV strains. The CMV-specific CTL response was class I major histocompatibility complex restricted and mediated by CD8+ cells. An early CMV protein(s) was the dominant target recognized by bulk CTL, although the pattern of CTL recognition of CMV proteins varied among animals. Analysis of CMV-specific CTL responses in macaques should serve as a valuable model for CMV immunopathogenesis and will facilitate prospective in vivo studies of immune interactions between CMV and SIV in AIDS. |
| Databáze: | OpenAIRE |
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