OPNa overexpression is associated with matrix calcification in thyroid cancer cell lines
| Autor: | Manuel Sobrinho-Simões, Catarina Tavares, Etel Rodrigues Pereira Gimba, Paula Soares, Andreia M. Silva, Luciana Ferreira, Catarina Eloy, Ana Clara Santos da Fonseca Bastos, Raquel T. Lima, Elisabete Rios, Ana Pestana |
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| Přispěvatelé: | Instituto de Investigação e Inovação em Saúde |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male osteopontin Osteocalcin / metabolismo Time Factors RNA Messenger/metabolism lcsh:Chemistry 0302 clinical medicine Osteopontin Calcinosis / pathology lcsh:QH301-705.5 Thyroid cancer Spectroscopy Osteopontin / genetics biology Chemistry psammoma bodies Calcinosis General Medicine Middle Aged Extracellular Matrix 3. Good health Computer Science Applications Thyroid Neoplasms / metabolismo Thyroid Cancer Papillary 030220 oncology & carcinogenesis Thyroid Neoplasms / pathology papillary thyroid carcinoma Female Collagen medicine.symptom Psammoma body Osteocalcin Osteopontin / metabolismo OPNa Article Catalysis Inorganic Chemistry Thyroid carcinoma 03 medical and health sciences Dystrophic calcification stomatognathic system medicine matrix calcification Humans Thyroid Cancer Papillary / metabolismo RNA Messenger Thyroid Neoplasms Gene Silencing Physical and Theoretical Chemistry Molecular Biology RNA Messenger / genetics Staining and Labeling Thyroid Cancer Papillary / pathology Organic Chemistry Collagen / biosynthesis Cancer Calcinosis / metabolismo Extracellular Matrix / metabolismo medicine.disease Desmoplasia 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 biology.protein Cancer research Calcification |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP International Journal of Molecular Sciences Volume 19 Issue 10 International Journal of Molecular Sciences, Vol 19, Iss 10, p 2990 (2018) |
| Popis: | Osteopontin (OPN) spliced variants (OPN-SV: OPNa, OPNb, and OPNc) are aberrantly expressed in tumors and frequently associated with cancer progression. This holds true for papillary thyroid carcinoma (PTC), which is the most common type of thyroid cancer (TC). PTC often presents with desmoplasia and dystrophic calcification, including psammoma bodies (PB). This work aimed to investigate total OPN (tOPN) and OPN-SV expression and their association with the presence of PB in the PTC classical variants (cPTC), as well as the involvement of OPN-SV in matrix calcification of TC cell lines. We found that cPTC samples presenting PB showed higher OPN expression levels. In TC cell lines, OPNa overexpression promotes higher matrix calcification and collagen synthesis when compared to that of clones overexpressing OPNb or OPNc. In response to OPN knockdown, calcification was inhibited, paralleled with the downregulation of calcification markers. In conclusion, our data evidenced that OPN expression is associated with the presence of PB in cPTC samples. Among the OPN-SV, OPNa is the main contributor to matrix calcification in tested TC cells, providing clues to a better understanding on the biology and ethiopathogenesis of the calcification process in TC cells. This study was supported by the CNPq (grant n# 237322/2012-9); by FCT (grants n# SFRH/BD/87887/2012 and SFRH/BD/110617/2015); by FEDER “Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020”; by Operacional Programme for Competitiveness and Internationalization “POCI” (grant n# POCI-01-0145-FEDER-007274); by the “Advancing cancer research: from basic knowledge to application” (grand n# NORTE-01-0145-FEDER-000029); and by the “Projetos Estruturados de I & D & I”, funded by Norte 2020—Programa Operacional Regional do Norte. |
| Databáze: | OpenAIRE |
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