Topical treatments with acylceramide dispersions restored stratum corneum lipid lamellar structures in a reconstructed human epidermis model
| Autor: | Shigeki Kageyama, Gen Omura, Sugishima Akinori, Ichiro Hatta, Akina Nakaune-Iijima, Tomoko Tashiro, Hiroyuki Kitaoka |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Skin barrier Skin permeability Ceramides Biochemistry Permeability Surface-Active Agents 03 medical and health sciences Microscopy Electron Transmission Pulmonary surfactant Scattering Small Angle Dry skin Stratum corneum medicine Humans Lamellar structure Molecular Biology Molecular Structure integumentary system Small-angle X-ray scattering Chemistry Organic Chemistry Cell Biology Lipids body regions 030104 developmental biology medicine.anatomical_structure Epidermal Cells Biophysics Epidermis medicine.symptom |
| Zdroj: | Chemistry and Physics of Lipids. 215:56-62 |
| ISSN: | 0009-3084 |
| DOI: | 10.1016/j.chemphyslip.2018.05.003 |
| Popis: | Long-periodicity phase (LPP) lamellar structures in intercellular lipid matrixes of the stratum corneum (SC) are considered important for maintenance of skin permeability barriers. Acylceramides are essential components of LPP structures, and their absence influences skin barriers under physiological and pathological conditions, such as atopic dermatitis and dry skin. Although topical applications of acylceramide have been shown to facilitate maintenance of the skin barrier, it is unknown whether topically applied acylceramides are incorporated into intercellular lipids to form LPP structures. Thus, we assessed the effects of topical treatments with monomodal acylceramides on the formation of LPP structures in a surfactant-insulted reconstructed human epidermis model using small-angle X-ray scattering (SAXS) and transmission electron microscopy (TEM) analyses. In SAXS experiments, LPP structures give rise to a diffraction peak which indicates the presence of a structure with a 13 nm real space repeat distance. LPP patterns of intercellular lipid matrixes in the SC were disrupted’ by surfactant treatments and were recovered by topical acylceramide treatments. TEM images also showed specific repeating patterns of LPP structures, indicating that topical acylceramide treatments facilitate recovery of LPP structures in the SC. The present data show that the application of acylceramides might temporarily modify the lipid structure to resemble that of normal skin although the underlying cause of dry or diseased skin is not fully clarified. |
| Databáze: | OpenAIRE |
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