Mass spectrometry imaging of phosphatidylcholine metabolism in lungs administered with therapeutic surfactants and isotopic tracers
| Autor: | Emily Hall, Shane R. Ellis, Jens Koed Madsen, Ron M. A. Heeren, Madhuriben H. Panchal, Howard Clark, Anthony D. Postle, Grielof Koster, Bryn Flinders |
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| Přispěvatelé: | Imaging Mass Spectrometry (IMS), RS: M4I - Imaging Mass Spectrometry (IMS) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
ELECTROSPRAY-IONIZATION CLEARANCE MSI Mass spectrometry imaging Endogeny 030204 cardiovascular system & hematology Tandem mass spectrometry Biochemistry chemistry.chemical_compound 0302 clinical medicine Endocrinology Pulmonary surfactant isotope labeling ALVEOLAR MACROPHAGES PLA1 Phospholipase A1 remodeling PROTEIN-A ATII Alveolar epithelial type II EtOH Ethanol DPPC Dipalmitoyl phosphatidylcholine TURNOVER ARACHIDONIC-ACID METABOLISM Research Article PC Phosphatidylcholine TISSUES Resolution (mass spectrometry) QD415-436 PLA2 Phospholipase A2 Mass spectrometry imaging lipids 03 medical and health sciences Surface-Active Agents MS/MS Tandem mass spectrometry Phosphatidylcholine Labelling BALF Bronchoalveolar lavage fluid KINETICS matrix-assisted laser desorption/ionization Lipid metabolism Cell Biology Metabolism CHCL3 Chloroform MeOH Methanol Matrix-assisted laser desorption/ionization 030104 developmental biology chemistry PHOSPHOLIPID-COMPOSITION SIMS Secondary ion mass spectrometry |
| Zdroj: | Journal of Lipid Research, 62:100023. American Society for Biochemistry and Molecular Biology, Inc. Journal of Lipid Research Journal of Lipid Research, Vol 62, Iss, Pp 100023-(2021) |
| ISSN: | 0022-2275 |
| Popis: | Mass spectrometry imaging (MSI) visualises molecular distributions throughout tissues but is blind to dynamic metabolic processes. Here, MSI with high mass resolution together with multiple stable isotope labelling provided spatial analyses of phosphatidylcholine (PC) metabolism in mouse lungs. Dysregulated surfactant metabolism is central to many respiratory diseases. Metabolism and turnover of therapeutic pulmonary surfactants were imaged from distributions of intact and metabolic products of an added tracer, universally13C-labelled dipalmitoyl PC (U[13C]DPPC). The parenchymal distributions of newly synthesised PC species were also imaged from incorporations ofmethyl-D9-choline. This dual labelling strategy demonstrated both lack of inhibition of endogenous PC synthesis by exogenous surfactant and location of acyl chain remodelling processes acting on the U[13C]DPPC-labelled surfactant, leading to formation of polyunsaturated PC lipids. This ability to visualise discrete metabolic events will greatly enhance our understanding of lipid metabolism in diverse tissues, and has potential application to both clinical and experimental studies. |
| Databáze: | OpenAIRE |
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