Expression of band 3 anion exchanger induces chloride current and taurine transport: structure-function analysis
| Autor: | F Borgese, R Motais, Nicole Gabillat, B. Fievet |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Taurine
Trout Recombinant Fusion Proteins Xenopus Molecular Sequence Data Biological Transport Active Biology Chloride Ion Channels General Biochemistry Genetics and Molecular Biology Mice chemistry.chemical_compound Chlorides Anion Exchange Protein 1 Erythrocyte medicine Animals Amino Acid Sequence Molecular Biology Band 3 Taurine transport Ion channel Chloride channel activity Base Sequence General Immunology and Microbiology General Neuroscience Gene Transfer Techniques biology.organism_classification chemistry Biochemistry biology.protein Biophysics Gene Deletion Intracellular Research Article medicine.drug |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0261-4189 |
| DOI: | 10.1002/j.1460-2075.1995.tb00200.x |
| Popis: | Most, but not all, cell types release intracellular organic solutes (e.g. taurine) in response to cell swelling to achieve cell volume regulation. Although this efflux is blocked by classical inhibitors of the electroneutral anion exchanger band 3 (AE1), it is thought to involve an anion channel. The role of band 3 in volume-dependent taurine transport was determined by expressing, in Xenopus oocytes, band 3 from erythrocytes which do (trout) or do not (mouse) release taurine when swollen. AE1 of both species elicited anion exchange activity, but only trout band 3 showed chloride channel activity and taurine transport. Chimeras constructed from trout and mouse band 3 allowed the identification of some protein domains critically associated with channel activity and taurine transport. The data provide evidence that swelling-induced taurine movements occur via an anion channel which is dependent on, or controlled by, band 3. They suggest the involvement of proteins of the band 3 (AE) family in cell volume regulation. |
| Databáze: | OpenAIRE |
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