The adoptive transfer of BCG-induced T lymphocytes contributes to hippocampal cell proliferation and tempers anxiety-like behavior in immune deficient mice
| Autor: | Zhi Bin Yao, Zhongsheng Tang, ShuaiShuai Liu, Jinhai Duan, Fangfang Qi, Dan Song |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adoptive cell transfer Hippocampus Social Sciences Anxiety White Blood Cells Mice 0302 clinical medicine Animal Cells Medicine and Health Sciences Cytotoxic T cell Psychology Public and Occupational Health Lymphocytes L-Selectin Mammals Multidisciplinary Animal Behavior T Cells Neurogenesis Eukaryota Animal Models Vaccination and Immunization Adoptive Transfer medicine.anatomical_structure Hyaluronan Receptors Experimental Organism Systems Cell Processes Vertebrates BCG Vaccine Medicine Cellular Types Research Article Elevated plus maze Science T cell Immune Cells Immunology Mice Nude Spleen Mouse Models Cytotoxic T cells Biology Research and Analysis Methods Rodents 03 medical and health sciences Interferon-gamma Immune system Model Organisms medicine Animals Cell Proliferation Behavior Blood Cells Cell growth Organisms Biology and Life Sciences Cell Biology 030104 developmental biology Animals Newborn Gene Expression Regulation Amniotes Animal Studies Clinical Immunology Preventive Medicine Interleukin-4 Clinical Medicine Zoology 030217 neurology & neurosurgery CD8 |
| Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 4, p e0225874 (2020) |
| ISSN: | 1932-6203 |
| Popis: | 1.AbstractWe previously have reported that neonatal Bacillus Calmette-Guerin (BCG) vaccination improves neurogenesis and behavior in early life through affecting the neuroimmune milieu in the brain, but it is uncertain whether activation phenotypes and functional changes in T lymphocytes shape brain development. Here, we studied the effects of BCG vaccination via the adoptive transfer of T lymphocytes from the BALB/c wild-type mice into naive mice. Our results show that mice adoptive BCG-induced lymphocytes (BCG->naive mice) showed anxiolytic and antidepressant-like performance when completing an elevated plus maze (EPM) test. Meanwhile, BCG->naive mice possess more cell proliferation and newborn neurons than PBS->naive and nude mice in the hippocampus. IFN-γ and IL-4 levels in the serum of BCG->naive mice also increased, while TNF-α and IL-1β levels were reduced relative to those of PBS->naive and nude mice. We further found that BCG->naive mice showed different repartition of CD4+ and CD8+ T cell to naive (CD62L+ CD44low), effector memory (CD62L− CD44hi), central memory (CD62L+ CD44hi) and acute/activated effector (CD62L− CD44low) cells in the spleen. Importantly, the adoptive transfer of BCG-induced T lymphocytes infiltrated into the dura mater and brain parenchyma of the nude mice. Activation phenotypes and functional changes in T lymphocytes are very likely to affect the neuroimmune milieu in the brain, and alterations in ratios of splenic CD4+ and CD8+ memory T cells may affect the expression of correlative cytokines in the serum, accounting for our behavioral results. We conclude thus that the adoptive transfer of BCG-induced T lymphocytes contributes to hippocampal cell proliferation and tempers anxiety-like behavior in immune deficient mice. Our work shows that BCG vaccination improves hippocampal cell proliferation outcomes and behaviors, likely as a result of splenic effector/memory T lymphocytes regulating the neuroimmune niche in the brain. |
| Databáze: | OpenAIRE |
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