Toxicological Responses of α-Pinene-Derived Secondary Organic Aerosol and Its Molecular Tracers in Human Lung Cell Lines
| Autor: | Yuzhi Chen, Agata Kołodziejczyk, Alicia Martínez-Romero, Jason D. Surratt, Faria Khan, Rafal Szmigielski, Yue Zhang, Andrew T. Lambe, Krzysztof J. Rudzinski, Nasir Jalal, Rebecca C. Fry, Karina Kwapiszewska |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Fine particulate
Cell Survival 010501 environmental sciences Toxicology complex mixtures 01 natural sciences behavioral disciplines and activities Article Human lung 03 medical and health sciences chemistry.chemical_compound Human health medicine Humans Cells Cultured 030304 developmental biology 0105 earth and related environmental sciences Bicyclic Monoterpenes Cell Proliferation Aerosols 0303 health sciences Pinene Ozonolysis Inhalation Dose-Response Relationship Drug Molecular Structure Chemistry General Medicine Aerosol Oxidative Stress medicine.anatomical_structure 13. Climate action Environmental chemistry |
| Zdroj: | Chemical Research in Toxicology |
| ISSN: | 1520-5010 0893-228X |
| Popis: | Secondary organic aerosol (SOA) is a major component of airborne fine particulate matter (PM2.5) that contributes to adverse human health effects upon inhalation. Atmospheric ozonolysis of α-pinene, an abundantly emitted monoterpene from terrestrial vegetation, leads to significant global SOA formation; however, its impact on pulmonary pathophysiology remains uncertain. In this study, we quantified an increasing concentration response of three well-established α-pinene SOA tracers (pinic, pinonic, and 3-methyl-1,2,3-butanetricarboxylic acids) and a full mixture of α-pinene SOA in A549 (alveolar epithelial carcinoma) and BEAS-2B (bronchial epithelial normal) lung cell lines. The three aforementioned tracers contributed ∼57% of the α-pinene SOA mass under our experimental conditions. Cellular proliferation, cell viability, and oxidative stress were assessed as toxicological end points. The three α-pinene SOA molecular tracers had insignificant responses in both cell types when compared with the α-pinene SOA (up to 200 μg mL–1). BEAS-2B cells exposed to 200 μg mL–1 of α-pinene SOA decreased cellular proliferation to ∼70% and 44% at 24- and 48-h post exposure, respectively; no changes in A549 cells were observed. The inhibitory concentration-50 (IC50) in BEAS-2B cells was found to be 912 and 230 μg mL–1 at 24 and 48 h, respectively. An approximate 4-fold increase in cellular oxidative stress was observed in BEAS-2B cells when compared with untreated cells, suggesting that reactive oxygen species (ROS) buildup resulted in the downstream cytotoxicity following 24 h of exposure to α-pinene SOA. Organic hydroperoxides that were identified in the α-pinene SOA samples likely contributed to the ROS and cytotoxicity. This study identifies the potential components of α-pinene SOA that likely modulate the oxidative stress response within lung cells and highlights the need to carry out chronic exposure studies on α-pinene SOA to elucidate its long-term inhalation exposure effects. |
| Databáze: | OpenAIRE |
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