Genetic variability ofBaylisascaris schroederifrom the Qinling subspecies of the giant panda in China revealed by sequences of three mitochondrial genes
| Autor: | Yan-Qin Fang, Guang-Hui Zhao, Zhong-Hui Zhao, Wan-Xin Ren, Wen-Yu Cheng, Qing-Qing Bian, Yan-Qing Jia |
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| Rok vydání: | 2013 |
| Předmět: |
China
Mitochondrial DNA Genetic Speciation Molecular Sequence Data Zoology Baylisascaris Subspecies DNA Mitochondrial DNA sequencing Species Specificity Ascaridoidea Genetics Animals Cytochrome c oxidase Genetic variability Molecular Biology Genome Helminth Phylogenetic tree biology Cytochrome b Ecology Genetic Variation Sequence Analysis DNA Cytochromes b DNA Helminth biology.organism_classification Genes Mitochondrial biology.protein Ursidae |
| Zdroj: | Mitochondrial DNA. 25:212-217 |
| ISSN: | 1940-1744 1940-1736 |
| DOI: | 10.3109/19401736.2013.792074 |
| Popis: | The present study examined the variations in three mitochondrial (mt) DNA sequences, namely cytochrome b (cytb), cytochrome c oxidase subunit 3 (cox3) and NADH dehydrogenase subunit 5 (nad5), among Baylisascaris schroederi isolates from the Qinling subspecies of the giant panda in Shaanxi province, northwestern China. No differences in length were detected in the three mt fragments from different isolates. The intra-specific sequence variations within all B. schroederi samples were 0-2.6% for pcytb, 0-1.8% for pcox3 and 0-2.1% for pnad5, while the inter-specific sequence differences among members of the genus Baylisascaris were 8.2-15.2%, 6.2-15.9% and 8.4-16.0% for pcytb, pcox3, pnad5, respectively. A phylogenetic analysis of the combined sequences of pcytb, pcox3 and pnad 5 showed that all B. schroederi samples in the present study were located in two large clusters, with one cluster containing samples from giant pandas in Sichuan province. These findings provide basic information for further study of molecular epidemiology and control of B. schroederi infection in the Qinling subspecies of the giant panda and throughout China. |
| Databáze: | OpenAIRE |
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